Racaniello V R, Baltimore D
Science. 1981 Nov 20;214(4523):916-9. doi: 10.1126/science.6272391.
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
脊髓灰质炎病毒RNA基因组的完整克隆互补DNA拷贝构建于细菌质粒pBR322的Pst I位点。用这种杂交质粒转染培养的哺乳动物细胞可产生有传染性的脊髓灰质炎病毒。用缺乏脊髓灰质炎病毒基因组前115个碱基的质粒转染的细胞不产生病毒。
