通过MgCl₂处理从组蛋白激酶中分离出的大鼠和小鼠乳腺上皮细胞胞质溶胶中的酪蛋白激酶，以及妊娠和泌乳对该酶活性的影响。

Casein kinase in cytosol of rat and mouse mammary epithelial cells isolated from histone kinase by MgCl2 treatment and influence of pregnancy and lactation on the enzyme activity.

作者信息

Asaga H, Mutö K, Homareda H, Komine S

出版信息

Endocrinol Jpn. 1981 Jun;28(3):265-70. doi: 10.1507/endocrj1954.28.265.

DOI:10.1507/endocrj1954.28.265

Abstract

Rat mammary glands contain cyclic AMP-independent casein kinase and cyclic AMP-dependent histone kinase. The former was easily isolated from cyclic AMP-dependent histone kinase by MgCl2 treatment. Mammary casein kinase was not activated by cyclic nucleotides, and Mg++ and ATP were required for activation. The specific activity of casein kinase in cytosol of rat mammary epithelial cells increased 2 to 3-fold during pregnancy and lactation. Cytosol of mouse mammary epithelial cells also contained cyclic AMP-independent casein kinase, and the activity of this enzyme was about three times that of the Golgi fraction.

摘要

大鼠乳腺含有不依赖环磷酸腺苷（cAMP）的酪蛋白激酶和依赖环磷酸腺苷的组蛋白激酶。通过氯化镁（MgCl2）处理，前者很容易从依赖环磷酸腺苷的组蛋白激酶中分离出来。乳腺酪蛋白激酶不会被环核苷酸激活，其激活需要镁离子（Mg++）和三磷酸腺苷（ATP）。在怀孕和哺乳期间，大鼠乳腺上皮细胞胞质溶胶中酪蛋白激酶的比活性增加了2至3倍。小鼠乳腺上皮细胞的胞质溶胶中也含有不依赖环磷酸腺苷的酪蛋白激酶，这种酶的活性约为高尔基体部分的三倍。