Müller P K, Raisch K, Kadner H, Wiedemann H
Hoppe Seylers Z Physiol Chem. 1978 Jan;359(1):13-7. doi: 10.1515/bchm.1978.359.1.13.
A precursor of type I collagen was accumulated when cranial bones from embryonic chicken were cultured in vitro in the presence of 2,2'-bipyridyl. While biochemical studies demonstrate that the precursor was present as a covalently linked trimer, evaluation of electron micrographs of segment-long-spacing aggregates show that the molecules of this precursor have an extension at the carboxy terminus only. These data provide further evidence that the inter-chain disulfide bonds in procollagen are located at the carboxy-terminal extension, and secondly, that 2,2'-bipyridyl does not interfere with a normal cleavage of procollagen peptides at the amino terminus, but inhibits the cleavage of the carboxy-terminal procollagen peptides.
