Schmidt W A, Klein M, Brade L
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981;250(1-2):25-33.
We examined the practical value of the Latex-IgM-test in diagnostic virology. Sera of patients suspected to have a cytomegalic infection were used for serological investigation. Cytomegalovirus produced in cell cultures was incubated with serum from patients. The resulting virus-antibody complex was removed by centrifugation and sensibilized Latex particles were added (anti IgG and anti IgM). The quantity and type of the bound antibody could be determined in this way. The sera were checked by parallel determination by another reliable method, the neutralization test. Immunoglobulins G and M separated and tested for antibodies. The usefulness of the Latex-IgM test for the routine diagnostics could be evaluated in the present study.
我们研究了乳胶IgM试验在诊断病毒学中的实用价值。疑似患有巨细胞感染患者的血清用于血清学研究。将细胞培养物中产生的巨细胞病毒与患者血清一起孵育。通过离心去除产生的病毒-抗体复合物,然后加入致敏乳胶颗粒(抗IgG和抗IgM)。通过这种方式可以确定结合抗体的数量和类型。血清通过另一种可靠方法即中和试验进行平行测定来检查。分离免疫球蛋白G和M并检测抗体。在本研究中可以评估乳胶IgM试验在常规诊断中的实用性。