Miller H, McCulloch B, Landini M P, Rossier E
Regional Virology Laboratory, Children's Hospital of Eastern Ontario, Ottawa, Canada.
J Clin Microbiol. 1989 Dec;27(12):2672-7. doi: 10.1128/jcm.27.12.2672-2677.1989.
Sequential specimens from nine allograft recipients were examined by using a variety of methods to detect primary cytomegalovirus (CMV) infection as rapidly as possible posttransplantation. Sera were examined for immunoglobulin G (IgG) and IgM antibodies by immunoblotting, enzyme immunoassay, and immunofluorescence and also by complement fixation, latex agglutination, and an immunofluorescence test for antibody to CMV early antigen. Urine and occasionally blood, tissue, and other specimens were centrifuged onto cell cultures to enhance CMV infectivity. Eight of the nine patients showed laboratory evidence of primary CMV infection, and CMV was isolated from seven of the eight: in no case was virus isolated before seroconversion had become evident. However, serological tests differed in their abilities to detect antibody response to CMV infection in different patients; while immunoblotting, latex agglutination, and enzyme immunoassay for IgG antibodies generally detected seroconversion before complement fixation, this was not invariably the case. At present, optimal laboratory detection of CMV infections in these patients can be achieved only by a combination of serological methods and virus isolation.
采用多种方法对9例同种异体移植受者的系列标本进行检测，以便在移植后尽快检测出原发性巨细胞病毒（CMV）感染。通过免疫印迹法、酶免疫测定法和免疫荧光法，以及补体结合试验、乳胶凝集试验和CMV早期抗原抗体免疫荧光试验检测血清中的免疫球蛋白G（IgG）和IgM抗体。将尿液以及偶尔的血液、组织和其他标本离心接种到细胞培养物上，以提高CMV的感染性。9例患者中有8例显示出原发性CMV感染的实验室证据，8例中的7例分离出了CMV：在血清转化明显之前，无一例分离出病毒。然而，血清学检测在检测不同患者对CMV感染的抗体反应能力方面存在差异；虽然免疫印迹法、乳胶凝集试验和IgG抗体酶免疫测定法通常比补体结合试验更早检测到血清转化，但情况并非总是如此。目前，只有通过血清学方法和病毒分离相结合，才能实现对这些患者CMV感染的最佳实验室检测。
