Marquardt W W, Snyder D B, Schlotthober B A
Avian Dis. 1981 Jul-Sep;25(3):713-22.
An enzyme-linked immunosorbent assay (ELISA) for detecting and quantifying antibodies to infectious bronchitis virus (IBV) is described. Purified antigen, prepared on sucrose density gradients, was required to decrease the nonspecific background, and saline was found to be superior to bicarbonate buffer for coating the cuvettes with antigen. The sensitivity of the test in measuring antiserum titers could be altered greatly and linearly by adjusting the protein content of the antigen. The ELISA was able to detect an antibody response to IBV infection earlier than the virus-neutralization (VN) test. Antibody titers obtained by ELISA were considerably higher than those obtained by VN. Serotypes of IBV could not be differentiated with ELISA because of extensive antiserum cross-reactivity. The utility of ELISA in studies on IBV is discussed.
本文描述了一种用于检测和定量传染性支气管炎病毒（IBV）抗体的酶联免疫吸附测定（ELISA）。需要使用在蔗糖密度梯度上制备的纯化抗原以降低非特异性背景，并且发现用生理盐水包被比色杯的抗原效果优于碳酸氢盐缓冲液。通过调整抗原的蛋白质含量，该检测方法在测量抗血清滴度时的灵敏度可大幅线性改变。ELISA能够比病毒中和（VN）试验更早地检测到对IBV感染的抗体反应。ELISA获得的抗体滴度明显高于VN试验获得的滴度。由于抗血清广泛交叉反应，ELISA无法区分IBV的血清型。文中讨论了ELISA在IBV研究中的实用性。
