聚（ADP-核糖）在体内具有分支结构。

Poly(ADP-ribose) has a branched structure in vivo.

作者信息

Juarez-Salinas H, Levi V, Jacobson E L, Jacobson M K

出版信息

J Biol Chem. 1982 Jan 25;257(2):607-9.

Abstract

We have searched for the presence of branching in the chromosomal polymer poly(ADP-ribose) as it occurs in vivo. Treatment of the polymer with phosphodiesterase asnd phosphomonoesterase results in the conversion of internal residues to the nucleoside ribosyladenosine and the conversion of points of branching to diribosyladenosine. We have detected diribosyladenosine in digests of the polymer derived from carcinogen-treated SV40 virus-formed 3T3 cells and in normal rat liver, kidney, and spleen. The frequency of residues involved in branching varied from 0.8 to 1.6 mole % over a 50-fold range of total levels of poly(ADP-ribose). Thus, branching seems to be a general feature of poly(ADP-ribose) as it occurs in vivo.

摘要

我们已经在体内存在的染色体聚合物聚（ADP - 核糖）中寻找分支的存在。用磷酸二酯酶和磷酸单酯酶处理该聚合物会导致内部残基转化为核苷核糖基腺苷，并且分支点转化为二核糖基腺苷。我们已经在源自致癌物处理的SV40病毒形成的3T3细胞以及正常大鼠肝脏、肾脏和脾脏的聚合物消化物中检测到二核糖基腺苷。在聚（ADP - 核糖）总水平的50倍范围内，参与分支的残基频率从0.8到1.6摩尔％不等。因此，分支似乎是体内聚（ADP - 核糖）的一个普遍特征。