Paddon H B, Vigo C, Vance D E
Biochim Biophys Acta. 1982 Jan 15;710(1):112-5. doi: 10.1016/0005-2760(82)90197-7.
Studies have been performed on the mechanism by which diethylstilbestrol stimulates the activity of choline kinase in livers from cockerels. The enzyme was purified 700-900-fold by affinity chromatography. The increased enzyme activity could not be accounted for by diethylstilbestrol alteration of the kinetic constants of the enzyme. Rabbit antibody was raised to the purified enzyme. Titration studies with antiserum demonstrated a 2-fold increase in the amount of choline kinase in diethylstilbestrol-treated cytosol, which correlated with a 2-fold elevation of the activity of the enzyme. We conclude that diethylstilbestrol stimulates the activities of choline kinase in cockerel liver by corresponding increase in the amount of enzyme.
针对己烯雌酚刺激小公鸡肝脏中胆碱激酶活性的机制开展了多项研究。通过亲和层析法将该酶纯化了700 - 900倍。己烯雌酚对该酶动力学常数的改变无法解释酶活性的增加。制备了针对纯化酶的兔抗体。用抗血清进行的滴定研究表明，己烯雌酚处理的细胞溶质中胆碱激酶的量增加了2倍，这与该酶活性升高2倍相关。我们得出结论，己烯雌酚通过相应增加酶的量来刺激小公鸡肝脏中胆碱激酶的活性。
