Shimotohno K, Temin H M
Cell. 1981 Oct;26(1 Pt 1):67-77. doi: 10.1016/0092-8674(81)90034-9.
We have prepared several infectious stocks of an avian retrovirus, spleen necrosis virus, containing the herpes simplex virus type 1 thymidine kinase (tk) gene. The viruses were produced after cotransfection of chicken cells with DNA from recombinants between cloned spleen necrosis virus and tk DNAs and DNA of cloned reticuloendotheliosis virus strain A. removal of sequences in the tk gene for the end of tk mRNA increased a thousand fold the yield of infectious recombinant virus. Infection of chicken or rat tk- cells with the recombinant virus transformed them to a tk+ phenotype.
我们制备了几种禽逆转录病毒——脾坏死病毒的感染性毒株，其含有单纯疱疹病毒1型胸苷激酶（tk）基因。这些病毒是通过将鸡细胞与克隆的脾坏死病毒和tk DNA之间的重组体DNA以及克隆的网状内皮组织增殖病病毒A株的DNA共转染后产生的。去除tk基因中tk mRNA末端的序列，使感染性重组病毒的产量提高了一千倍。用重组病毒感染鸡或大鼠的tk-细胞，可将它们转化为tk+表型。
