Iannaccone P M, Gardner R L, Harris H
J Cell Sci. 1978 Feb;29:249-69. doi: 10.1242/jcs.29.1.249.
Tumours were induced by chemical carcinogens in chimaeric mice made by the amalgamation of two embryos each producing a different electrophoretic variant of the enzyme glucose phosphate isomerase. Although, in these chimaeras, the smallest samples of normal tissue that could be analysed almost invariably contained both isoenzymes, almost all the tumours contained only one. An analysis of the size of the clones that formed the chimaeric epidermis permitted the conclusion that the epidermal tumours produced could not have risen from more than 8 cells; and the most probable interpretation of the data is that these tumours were clonal growths.
通过将两个胚胎融合制成嵌合小鼠，这两个胚胎分别产生磷酸葡萄糖异构酶的不同电泳变体，然后用化学致癌物诱导肿瘤形成。尽管在这些嵌合体中，几乎总能分析到的最小正常组织样本都含有两种同工酶，但几乎所有肿瘤只含有一种。对构成嵌合表皮的克隆大小进行分析后得出结论，所产生的表皮肿瘤不可能起源于超过8个细胞；对这些数据最合理的解释是，这些肿瘤是克隆性生长。
