Schmidt G H, Blount M A, Ponder B A
Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK.
Development. 1987 Jul;100(3):535-41. doi: 10.1242/dev.100.3.535.
The clonal organization of chimaeric mouse epidermis was demonstrated by in situ staining of whole-mount preparations using monoclonal antibodies directed against H-2k and H-2b antigens. A striking pattern of transversely oriented stripes or patches was found which extended from mid-dorsum to the flank region. The orientation of these patches indicates a preferred directional expansion of clones during the development of dorsal/lateral epidermis. The clonal pattern of the belly region differed in that stripes were not found, but a marked ventral midline boundary was observed. This demarcation line may be due to a physical effect, i.e. isolation of the left and right ventral halves of the epidermis during early embryogenesis with relatively little cell mingling following closure of the abdominal wall. The obvious nonhomogenous distribution of chimaeric components in dorsal/lateral and ventral epidermis contradicts assumptions of homogenous, fine-grained patchiness derived from electrophoretic analysis of tissue samples and used in studies of skin carcinogenesis. The observation that hair follicles may contain cells of both parental genotypes implies a polyclonal origin. Epidermal proliferative units as described by Potten (1974) were not revealed by the pattern of mosaicism at the cellular level in these chimaeric tissue sheets. This indicates that the proliferative compartment of each putative epidermal unit is polyclonal.
通过使用针对H-2k和H-2b抗原的单克隆抗体对整装制剂进行原位染色，证明了嵌合小鼠表皮的克隆组织。发现了一种从背部中部延伸到侧翼区域的横向条纹或斑块的显著模式。这些斑块的方向表明在背侧/外侧表皮发育过程中克隆有优先的定向扩展。腹部区域的克隆模式不同，未发现条纹，但观察到明显的腹侧中线边界。这条分界线可能是由于物理效应，即在胚胎早期发育过程中表皮左右腹侧两半被隔离，腹壁闭合后细胞混合相对较少。嵌合成分在背侧/外侧和腹侧表皮中明显的非均匀分布与从组织样本电泳分析得出并用于皮肤癌发生研究的均匀、细粒度斑块的假设相矛盾。毛囊可能包含两种亲代基因型细胞的观察结果意味着其起源是多克隆的。在这些嵌合组织片的细胞水平上，Potten（1974年）描述的表皮增殖单位未通过镶嵌模式显示出来。这表明每个假定的表皮单位的增殖区室是多克隆的。
