Farookhi R
Endocrinology. 1982 Mar;110(3):1061-3. doi: 10.1210/endo-110-3-1061.
The hallmark of the preovulatory follicles in the rat ovary appears to be the presence of receptors for both LH and FSH on follicular granulosa cells. We have tested the possibility that both gonadotropin receptors could share a common adenylate cyclase system utilising cell fusion techniques. Adenylate cyclase (and concomitant stimulation of progesterone synthesis) was inactivated in granulosa cells possessing both FSH and LH receptors by incubation of the cells with N-ethylmaleimide. This treatment did not affect hormone binding to the cells. Subsequent fusion, using polyethylene glycol, of the cyclase-inactivated cells with granulosa cells possessing FSH receptors only and an active cyclase restored LH stimulation of cAMP and progesterone production. These findings support the hypothesis that the LH and FSH receptors on granulosa cells of preovulatory follicles share a common adenylate cyclase system.
大鼠卵巢中排卵前卵泡的标志似乎是卵泡颗粒细胞上同时存在促黄体生成素（LH）和促卵泡生成素（FSH）的受体。我们利用细胞融合技术测试了两种促性腺激素受体可能共享一个共同的腺苷酸环化酶系统的可能性。通过将细胞与N-乙基马来酰亚胺孵育，在同时拥有FSH和LH受体的颗粒细胞中，腺苷酸环化酶（以及随之而来的孕酮合成刺激）被灭活。这种处理并不影响激素与细胞的结合。随后，使用聚乙二醇将腺苷酸环化酶失活的细胞与仅拥有FSH受体且腺苷酸环化酶活性正常的颗粒细胞进行融合，恢复了LH对环磷酸腺苷（cAMP）和孕酮产生的刺激。这些发现支持了这样一种假说，即排卵前卵泡颗粒细胞上的LH和FSH受体共享一个共同的腺苷酸环化酶系统。
