Field J B, Chou M C, Titus G, Worden W
Endocrinology. 1982 Mar;110(3):820-4. doi: 10.1210/endo-110-3-820.
An initial incubation of bovine thyroid slices with TSH causes decreased responsiveness to the subsequent addition of the hormone when the adenylate cyclase -cAMP system and other metabolic parameters are measured. After the initial incubation with TSH, refractoriness persists despite incubation of thyroid slices for 24 h in the absence of added TSH. Removal of persistently bound TSH by trypsin or antibody to TSH did not reverse the refractoriness during a subsequent 2 h incubation without added TSH. However, normal TSH responsivity was restored by the removal of TSH bound during the first incubation by the addition of either trypsin or antibody to TSH at the beginning of a 24-h second incubation. Restitution of TSH responsiveness after treatment with trypsin or antibody to TSH requires new protein synthesis. While TSH-induced refractoriness does not modify stimulation of cAMP by cholera toxin, its effect on glucose oxidation is significantly diminished. Menadiol stimulation of glucose oxidation is not inhibited in thyroid slices refractory to TSH. Thus, the effect of menadiol is subsequent to the block induced by TSH, whereas that of cholera toxin is proximal to it.
当测定腺苷酸环化酶 - 环磷酸腺苷（cAMP）系统及其他代谢参数时，用促甲状腺激素（TSH）对牛甲状腺切片进行初始孵育，会导致其对随后添加的该激素的反应性降低。在用TSH进行初始孵育后，即便在不添加TSH的情况下将甲状腺切片孵育24小时，不应性仍然存在。在随后不添加TSH的2小时孵育过程中，用胰蛋白酶或TSH抗体去除持续结合的TSH并不能逆转不应性。然而，在24小时的第二次孵育开始时添加胰蛋白酶或TSH抗体，去除第一次孵育期间结合的TSH后，可恢复正常的TSH反应性。用胰蛋白酶或TSH抗体处理后TSH反应性的恢复需要新的蛋白质合成。虽然TSH诱导的不应性不会改变霍乱毒素对cAMP的刺激作用，但其对葡萄糖氧化的影响会显著减弱。在对TSH不应的甲状腺切片中，甲萘醌对葡萄糖氧化的刺激作用未受抑制。因此，甲萘醌的作用发生在TSH诱导的阻断之后，而霍乱毒素的作用则与之相近。
