Janecki A, Lukaszyk A, Jakubowiak A
Folia Histochem Cytochem (Krakow). 1981;19(3):135-42.
The effects of growing of Sertoli cells isolated from rat seminiferous epithelium by a modified procedure, and responses of these cells to dBcAMP or FSH stimulation was estimated using morphological methods. The modified isolation procedure included repeated mechanical rinsing of tubule pieces with a modified EDTA containing Hanks medium. Moreover, streptornase instead DNase was added to the trypsine containing medium and this resulted in a better dispersion of tubule components. The culture conditions remained unmodified. A high degree homogeneity of the cultured cell population and an evident reactivity of these cells to dBcAMP and FSH was achieved. Furthermore, an observation of giant cells, visible in monolayer among the typical Sertoli cells, is discussed in this paper. Contrary to small number of myoid cells, being survived in the culture, these giant cells did not show positive reaction to alkaline phosphatase.
采用改良方法从大鼠生精上皮分离支持细胞，并使用形态学方法评估这些细胞对二丁酰环磷腺苷（dBcAMP）或促卵泡激素（FSH）刺激的反应。改良的分离程序包括用含有改良乙二胺四乙酸（EDTA）的汉克斯培养基对肾小管片段进行反复机械冲洗。此外，在含胰蛋白酶的培养基中添加链霉蛋白酶而非脱氧核糖核酸酶（DNase），这使得肾小管成分的分散性更好。培养条件保持不变。培养的细胞群体具有高度同质性，且这些细胞对dBcAMP和FSH具有明显反应性。此外，本文还讨论了在典型支持细胞单层中可见的巨细胞观察结果。与培养中存活的少量肌样细胞相反，这些巨细胞对碱性磷酸酶未显示阳性反应。
