Funke M, Felgner P, Geister R
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981 Dec;251(1):126-33.
Sera of 853 returnees from tropical countries, of 24 cases with amebic liver abscess and of 172 nonexposed German individuals were tested for antibodies to E. histolytica in the enzyme immunoassay (EIA). Antibody results were expressed as "multiple of normal activity (MONA)". The qualification of the EIA as a routine screening procedure for amebae specific antibodies was investigated and compared to that of the complement fixation test. Based on the symmetric frequency distribution of results from the 172 non-exposed controls the upper one sided limits for 95% (less than 2.8 MONA) and 99% (less than 4.2 MONA) specificity were determined. Routine results below the 95% specificity limit were considered "inconspicuous", such between both limits "borderline" and all MONA values exceeding the 99% specificity limit "conspicuous". The intention was to thereby secure a high degree of sensitivity for amebae antibody in the test. Cases with clinically confirmed liver abscess revealed a one sided lower 95% sensitivity limit (greater than or equal to 1.2 MONA), far above the onset of the defined sensitivity thresholds for conspicuous MONA values.
采用酶免疫测定法（EIA）检测了853名来自热带国家的归国人员、24例阿米巴肝脓肿患者以及172名未接触过相关病原体的德国人的血清中的溶组织内阿米巴抗体。抗体检测结果以“正常活性倍数（MONA）”表示。对EIA作为阿米巴特异性抗体常规筛查方法的适用性进行了研究，并与补体结合试验进行了比较。根据172名未接触过相关病原体的对照者检测结果的对称频率分布，确定了95%特异性（小于2.8 MONA）和99%特异性（小于4.2 MONA）的单侧上限。常规检测结果低于95%特异性上限的被视为“不显著”，介于两者之间的为“临界”，所有超过99%特异性上限的MONA值为“显著”。这样做的目的是确保检测中对阿米巴抗体具有高度敏感性。临床确诊为肝脓肿的病例显示，单侧下限95%敏感性界限（大于或等于1.2 MONA），远高于显著MONA值所定义的敏感性阈值起始点。
