Bystryn J C
J Immunol. 1978 Jan;120(1):96-101.
Soluble murine melanoma-associated antigens (MAA), partially purified from the media of B16 melanoma cells in culture by ammonium sulfate precipitation and Sephadex G-200 chromatography, were tested for their effect on tumor growth. MAA were immunogenic in syngenic mice as evidenced by their ability to induce anti-melanoma antibodies and partial protective tumor immunity. The level of immunity was variable. It ranged from retardation of tumor development to almost complete suppression of tumor growth. The results were influenced by the nature of the control group, since immunization to either normal tissue antigens or complete Freund's adjuvant enhanced tumor growth. Overall, 46 of 91 MAA immunized mice, but none of 114 control mice, survived over 6 weeks (p less than 0.001). The protective immunity was specific since MAA immunized mice were not resistant to challenge with an unrelated syngeneic tumor (BW 10232 ADENOCARCINOMA). Partially purified normal tissue antigens were also immunogenic in syngeneic mice. They induced low levels of antibodies to, and enhanced the growth of, melanoma. These findings indicate that the culture media of melanoma cells contains tumor antigens that retain their biologic activity after partial purification, and that can induce specific, although only partial, protective immunity to melanoma.
通过硫酸铵沉淀和葡聚糖凝胶G - 200柱色谱法从培养的B16黑色素瘤细胞培养基中部分纯化得到的可溶性小鼠黑色素瘤相关抗原（MAA），对其对肿瘤生长的影响进行了测试。MAA在同基因小鼠中具有免疫原性，这可通过它们诱导抗黑色素瘤抗体的能力和部分保护性肿瘤免疫得以证明。免疫水平是可变的。其范围从肿瘤发育迟缓到几乎完全抑制肿瘤生长。结果受对照组性质的影响，因为对正常组织抗原或完全弗氏佐剂的免疫会增强肿瘤生长。总体而言，91只接受MAA免疫的小鼠中有46只存活超过6周，但114只对照小鼠中无一存活超过6周（p小于0.001）。保护性免疫是特异性的，因为接受MAA免疫的小鼠对用无关的同基因肿瘤（BW 10232腺癌）进行的攻击没有抵抗力。部分纯化的正常组织抗原在同基因小鼠中也具有免疫原性。它们诱导产生低水平的抗体，并促进黑色素瘤的生长。这些发现表明，黑色素瘤细胞的培养基含有肿瘤抗原，这些抗原在部分纯化后仍保留其生物活性，并且可以诱导对黑色素瘤的特异性（尽管只是部分）保护性免疫。
