Ford M D, Houldsworth J, Lavin M F
Mutat Res. 1981 Dec;84(2):419-27. doi: 10.1016/0027-5107(81)90209-8.
The ability of a number of Epstein-Barr virus-transformed lymphoblastoid cells from ataxia telangiectasis (AT) patients to repair gamma-radiation damage to DNA was determined. All of these AT cells were previously shown to be hypersensitive to gamma-radiation. Two methods were used to determine DNA-repair synthesis: isopycnic gradient analysis and a method employing hydroxyurea to inhibit semiconservative DNA synthesis. Control, AT heterozygote and AT homozygote cells were demonstrated to have similar capacities for repair of radiation damage to DNA. In addition at high radiation doses (10-40 krad) the extent of inhibition of DNA synthesis was similar in the different cell types.
测定了一些来自共济失调毛细血管扩张症（AT）患者的爱泼斯坦-巴尔病毒转化的淋巴母细胞修复γ射线对DNA损伤的能力。所有这些AT细胞先前已被证明对γ射线高度敏感。使用了两种方法来测定DNA修复合成：等密度梯度分析和一种采用羟基脲抑制半保留DNA合成的方法。已证明对照细胞、AT杂合子细胞和AT纯合子细胞修复DNA辐射损伤的能力相似。此外，在高辐射剂量（10 - 40千拉德）下，不同细胞类型中DNA合成的抑制程度相似。
