Grossberger D, Clough W
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7271-5. doi: 10.1073/pnas.78.12.7271.
The Epstein-Barr virus (EBV)-induced intracellular DNA polymerase was assayed in vitro for the ability to utilize the mutagenic nucleotide analog 2-aminopurine deoxyribose triphosphate (d2apTP), incorporating it as the corresponding monophosphate into DNA or poly[d)(A-T)] template. Bacteriophage T4, lymphocyte alpha, and the EBV particle-associated DNA polymerases were assayed simultaneously for direct comparison. Unlike these three polymerases, which were capable of distinguishing between d2apTP and dATP with a strong preference for the latter, the EBV-induced DNA polymerase only weakly distinguished between dATP and d2apTP and incorporated substantial amounts of d2apTP into template. Detergent-treated lymphocyte nuclei undergoing a high level of EBV DNA synthesis were shown to incorporate the 2-aminopurine analog of dATP into viral DNA. The relative inability of the EBV-induced DNA polymerase to distinguish between the two purine nucleotides reported here is consistent with previous reports on the ready incorporation of other nucleotide analogs into DNA polymerases induced by other herpesviruses. Because most antiherpes agents currently in use or under study are nucleotide analogs, the viral mutagenic properties of these drugs should be examined.
对爱泼斯坦-巴尔病毒（EBV）诱导的细胞内DNA聚合酶进行了体外测定，以检测其利用诱变核苷酸类似物2-氨基嘌呤脱氧核糖三磷酸（d2apTP）的能力，即将其作为相应的单磷酸掺入DNA或聚[d(A-T)]模板中。同时对噬菌体T4、淋巴细胞α和EBV颗粒相关的DNA聚合酶进行了测定，以便直接比较。与这三种能够强烈区分d2apTP和dATP且更倾向于后者的聚合酶不同，EBV诱导的DNA聚合酶只能微弱地区分dATP和d2apTP，并将大量的d2apTP掺入模板中。经去污剂处理的淋巴细胞核在进行高水平的EBV DNA合成时，会将dATP的2-氨基嘌呤类似物掺入病毒DNA中。此处报道的EBV诱导的DNA聚合酶相对无法区分这两种嘌呤核苷酸，这与之前关于其他疱疹病毒诱导的DNA聚合酶能轻易将其他核苷酸类似物掺入其中的报道一致。由于目前正在使用或研究的大多数抗疱疹药物都是核苷酸类似物，因此应该检测这些药物的病毒诱变特性。
