不同哺乳动物组织中腺苷激酶的差异。

Differences of adenosine kinases from various mammalian tissues.

Yamada Y, Goto H, Ogasawara N

Comp Biochem Physiol B. 1982;71(3):367-72. doi: 10.1016/0305-0491(82)90396-0.

Abstract

Adenosine kinases purified to homogeneity from various mammalian tissues have a monomeric structure, and their molecular weights were estimated around 40,000. 2. The enzyme activity per wet weight of tissue appears to vary from source to source, but the specific activities of the final enzyme preparations were alike, which were 6.3--7.8 mumol/min/mg protein. 3. No or small difference was observed in the kinetic properties among all seven adenosine kinases. 4. Some differences in structure were observed among five liver enzymes from human, rabbit, rat, mouse and Mongolian gerbil, but no difference was observed between the enzymes from human placenta and liver, or the enzymes from rat liver and brain.

摘要

从各种哺乳动物组织中纯化至同质的腺苷激酶具有单体结构，其分子量估计约为40,000。2. 每单位组织湿重的酶活性似乎因来源而异，但最终酶制剂的比活性相似，为6.3--7.8微摩尔/分钟/毫克蛋白质。3. 在所有七种腺苷激酶的动力学性质中未观察到差异或差异很小。4. 在来自人、兔、大鼠、小鼠和长爪沙鼠的五种肝脏酶之间观察到一些结构差异，但在人胎盘和肝脏的酶之间，或大鼠肝脏和大脑的酶之间未观察到差异。