Mierendorf R C, Mueller G C
J Biol Chem. 1982 Apr 25;257(8):4496-500.
Plasmid subclones of the 5' and 3' regions of the beta major-globin gene have been used as probes to assay the level and character of the transcripts of this gene in differentiating Friend erythroleukemia cells treated with dexamethasone. Using an S1 nuclease protection protocol, it is shown that dexamethasone reduces the amount of precursor-specific sequences in differentiating Friend cells within 30 min without significantly affecting the nuclear turnover of these molecules. The sites of initiation and termination of transcription remained the same during the shutdown of transcription by the hormone. In contrast to the situation in Friend cells, dexamethasone failed to inhibit globin gene transcription in three normal erythroid tissues. The manner in which the Friend virus complex might render the globin genes sensitive to glucocorticoids is discussed.
已使用β-珠蛋白基因5'和3'区域的质粒亚克隆作为探针，来检测在用地塞米松处理的分化型弗氏红白血病细胞中该基因转录本的水平和特征。使用S1核酸酶保护实验方案表明，地塞米松在30分钟内可减少分化型弗氏细胞中前体特异性序列的量，而不会显著影响这些分子的核周转。在激素导致转录关闭期间，转录起始和终止位点保持不变。与弗氏细胞中的情况相反，地塞米松未能抑制三种正常红细胞组织中的珠蛋白基因转录。文中讨论了弗氏病毒复合物使珠蛋白基因对糖皮质激素敏感的方式。
