Goto N, Hirano N, Takahashi K, Fujiwara K
Jpn J Exp Med. 1981 Dec;51(6):345-54.
Cerebrocortical lesions produced in suckling rats by intracerebral inoculation with MHV-A59 was studied by electron microscopy. Many cytoplasmic vesicles and irregular patterns of the nuclear membrane were observed in infected neurons at 48 h post-inoculation (p.i.). The abnormally electron-lucent or -dense neurons were seen at 72 h p.i., and in the formers, many virions were detected between the outer and inner nuclear membranes as well as in the dilated endoplasmic reticulum cisternae. Both reticular and tubular inclusion bodies appeared in the cytoplasm and some virions seemed to be associated with an end of the latter. Many virions were also seen in the intercellular spaces. At 96 h p.i. many virions were present not only in the cytoplasm of degenerated neurons but also in astrocytes appearing in the spongy lesions. There was also degeneration of oligodendrocytes while having no virions. In rats which survived the acute phase of infection and were killed at 8 days or 14 days p.i., many macrophages and fibroblasts were accumulated in the thickened leptomeninges and submeningeal area. At 22 days p.i., atrophied electron-dense neurons having no virions were scattered throughout the cerebral cortex.
通过电子显微镜研究了乳鼠脑内接种MHV - A59所产生的大脑皮质病变。接种后48小时（p.i.），在受感染的神经元中观察到许多细胞质囊泡和核膜的不规则形态。接种后72小时，可见异常电子透亮或致密的神经元，在前者中，在外核膜和内核膜之间以及扩张的内质网池内检测到许多病毒粒子。网状和管状包涵体出现在细胞质中，一些病毒粒子似乎与后者的一端相关。在细胞间隙中也可见许多病毒粒子。接种后96小时，许多病毒粒子不仅存在于变性神经元的细胞质中，还存在于海绵状病变中出现的星形胶质细胞中。少突胶质细胞也有变性，但没有病毒粒子。在感染急性期存活并在接种后8天或14天处死的大鼠中，许多巨噬细胞和成纤维细胞积聚在增厚的软脑膜和脑膜下区域。接种后22天，无病毒粒子的萎缩性电子致密神经元散在于整个大脑皮质。
