Karnushina I L, Spatz M, Bembry J
Life Sci. 1982 Mar 8;30(10):849-58. doi: 10.1016/0024-3205(82)90599-9.
Cultured endothelial cells derived from cerebral microvessels separated from 2-day-old rat brain contain a specific beta 2 and alpha 2-adrenergic sensitive adenylate cyclase (AC). Among the various tested hormones, PGE1 and PGE2 were found to be the most potent activators, while adenosine, angiotensin I and II, gamma-aminobutyric acid and vasoactive intestinal peptide inhibited the enzyme activity. However, acetylcholine, histamine, serotonin, glycine, glutamine, bradykinin, neurotensin and vasopressin (Lysine and Arginine) had no effect on the adenylate cyclase activity in this model. The susceptibility of the cerebrovascular endothelial AC system to the vasoactive substances as well as presence of beta 2 and alpha 2-type adrenergic receptors in the cultured endothelium provides additional support for the proposed endothelial involvement in the regulation of cerebrovascular permeability and blood flow.
从2日龄大鼠脑中分离出的脑微血管来源的培养内皮细胞含有一种特定的β2和α2 - 肾上腺素能敏感腺苷酸环化酶（AC）。在各种测试的激素中，前列腺素E1和前列腺素E2被发现是最有效的激活剂，而腺苷、血管紧张素I和II、γ - 氨基丁酸和血管活性肠肽则抑制该酶的活性。然而，乙酰胆碱、组胺、5 - 羟色胺、甘氨酸、谷氨酰胺、缓激肽、神经降压素和血管加压素（赖氨酸和精氨酸）对该模型中的腺苷酸环化酶活性没有影响。脑血管内皮AC系统对血管活性物质的敏感性以及培养内皮中β2和α2型肾上腺素能受体的存在，为所提出的内皮细胞参与脑血管通透性和血流调节提供了额外支持。
