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高迁移率族非组蛋白HMG-20（泛素）和HMG-17对体外测定的组蛋白脱乙酰酶活性的影响。

Effect of high mobility group nonhistone proteins HMG-20 (ubiquitin) and HMG-17 on histone deacetylase activity assayed in vitro.

作者信息

Mezquita J, Chiva M, Vidal S, Mezquita C

出版信息

Nucleic Acids Res. 1982 Mar 11;10(5):1781-97. doi: 10.1093/nar/10.5.1781.

DOI:10.1093/nar/10.5.1781
PMID:6280157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320566/
Abstract

We have used a method previously described by Reeves and Candido (1) to partially release histone deacetylase from cell nuclei together with putative regulators of the enzyme. Histone deacetylase released from testis cell nuclei and its putative regulators were separated by gel filtration in Sepharose 6B. A peak of low molecular weight contains a heat-stable factor that stimulate histone deacetylase in vitro. Many of the properties of the activator coincide with those of the protein HMG-20 (ubiquitin). Ubiquitin isolated from testis cell nuclei stimulated histone deacetylase in vitro. It has been suggested that HMG-17 partially inhibits histone deacetylase in Fried cell nuclei (2). In our system, HMG-17 shows no inhibitory effect on histone deacetylase activity

摘要

我们采用了里夫斯和坎迪多之前描述的一种方法（1），使组蛋白脱乙酰酶与该酶的假定调节因子一起从细胞核中部分释放出来。从睾丸细胞核中释放的组蛋白脱乙酰酶及其假定调节因子通过在琼脂糖6B中进行凝胶过滤来分离。一个低分子量峰含有一种在体外刺激组蛋白脱乙酰酶的热稳定因子。该激活剂的许多特性与蛋白质HMG - 20（泛素）的特性一致。从睾丸细胞核中分离出的泛素在体外刺激组蛋白脱乙酰酶。有人提出HMG - 17在弗里德细胞核中部分抑制组蛋白脱乙酰酶（2）。在我们的系统中，HMG - 17对组蛋白脱乙酰酶活性没有抑制作用

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/cf1705e99f9f/nar00374-0364-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/69096c8f06a8/nar00374-0360-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/9a2902c2d8cd/nar00374-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/cf1705e99f9f/nar00374-0364-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/69096c8f06a8/nar00374-0360-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/9a2902c2d8cd/nar00374-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7da3/320566/cf1705e99f9f/nar00374-0364-b.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The identification of distinct populations of acetylated histone.乙酰化组蛋白不同群体的鉴定。
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Partial inhibition of histone deacetylase in active chromatin by HMG 14 and HMG 17.HMG 14和HMG 17对活性染色质中组蛋白脱乙酰酶的部分抑制作用。
Nucleic Acids Res. 1980 May 10;8(9):1947-63. doi: 10.1093/nar/8.9.1947.
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Structural studies on two high-mobility-group proteins from calf thymus, HMG-14 and HMG-20 (ubiquitin), and their interaction with DNA.对来自小牛胸腺的两种高迁移率族蛋白HMG - 14和HMG - 20（泛素）及其与DNA相互作用的结构研究。
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Physical-chemical properties of ubiquitin.
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Ubiquitin is the ATP-dependent proteolysis factor I of rabbit reticulocytes.泛素是兔网织红细胞中依赖ATP的蛋白水解因子I。
J Biol Chem. 1980 Aug 25;255(16):7529-32.
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High resolution acrylamide gel electrophoresis of histones.组蛋白的高分辨率丙烯酰胺凝胶电泳
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Histone acetylation by cell-free preparations from rat uterus: in vitro stimulation by estradiol-17 beta.大鼠子宫无细胞制剂的组蛋白乙酰化：17β-雌二醇的体外刺激作用
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Histone deacetylase from calf thymus.来自小牛胸腺的组蛋白脱乙酰酶。
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