Søvik O, Heiervang E, Aksnes L, Selvig S
Scand J Clin Lab Invest. 1981 Nov;41(7):669-74. doi: 10.3109/00365518109090513.
The effect of glucagon on plasma cyclic AMP (cAMP), insulin and blood glucose was examined in normal adult subjects. After an i.v. injection of glucagon there was a rapid, dose-dependent increase of plasma cAMP as well as insulin and blood glucose. Multiple injection of glucagon to the same subject with 60 min intervals gave almost identical responses of plasma cAMP and blood glucose, whereas the insulin response tended to decrease with time. Dose-dependent increases of plasma cAMP, insulin and blood glucose were also seen during a continuous i.v. infusion of glucagon. With the lowest doses of glucagon the blood glucose and plasma insulin concentrations were increased without any change of plasma cAMP. Plasma cAMP, insulin and blood glucose declined prior to the termination of glucagon infusion. During an endogenous hyperglucagonaemia, induced by alanine injection, there was no discernible change of plasma cAMP. We conclude that the early events of glucagon action may be studied in vivo by monitoring plasma cAMP. However, variations of plasma glucagon within the physiological range are not accompanied by measurable changes of cAMP in the peripheral circulation.
在正常成年受试者中研究了胰高血糖素对血浆环磷酸腺苷（cAMP）、胰岛素和血糖的影响。静脉注射胰高血糖素后，血浆cAMP以及胰岛素和血糖迅速出现剂量依赖性升高。以60分钟的间隔对同一受试者多次注射胰高血糖素，血浆cAMP和血糖的反应几乎相同，而胰岛素反应则随时间趋于降低。在持续静脉输注胰高血糖素期间，也观察到血浆cAMP、胰岛素和血糖的剂量依赖性升高。使用最低剂量的胰高血糖素时，血糖和血浆胰岛素浓度升高，而血浆cAMP无任何变化。在胰高血糖素输注结束前，血浆cAMP、胰岛素和血糖下降。在由丙氨酸注射诱导的内源性高胰高血糖素血症期间，血浆cAMP没有明显变化。我们得出结论，通过监测血浆cAMP可以在体内研究胰高血糖素作用的早期事件。然而，生理范围内血浆胰高血糖素的变化并未伴随外周循环中cAMP的可测量变化。
