不同去污剂对仙台病毒包膜溶解作用的一些特性
Some characteristics of Sendai virus envelope solubilization by different detergents.
作者信息
Tomas E, Samuel I, Istodor A, Bârnaure F, Kharitonenkov I, Zakomyrdin I
出版信息
Virologie. 1982 Jan-Mar;33(1):53-7.
Gel-filtration through Sepharose-2B of Sendai virus envelopes solubilized by Tween-20 or Triton X-100 treatment allows the separation of two elution peaks, hemagglutinin (HA) activity being detected only in the peak corresponding to large envelope fragments. Solubilization of the same virus envelopes with N-octylglycoside (NOG) results in a single elution peak, exhibiting both HA and neuraminidase activities. The results obtained suggest : 1) the similar envelope composition of the different virus populations, and 2) the different mechanisms of solubilization, in relation to the detergent used for disruption. The advantages of virus disruption by NOG are discussed.
用吐温 - 20 或 Triton X - 100 处理溶解的仙台病毒包膜通过琼脂糖 - 2B 进行凝胶过滤，可分离出两个洗脱峰，仅在对应于大包膜片段的峰中检测到血凝素（HA）活性。用 N - 辛基糖苷（NOG）溶解相同的病毒包膜会产生一个单一的洗脱峰，同时表现出血凝素和神经氨酸酶活性。所得结果表明：1）不同病毒群体的包膜组成相似；2）与用于破坏的去污剂相关的不同溶解机制。讨论了用 NOG 破坏病毒的优点。
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