de Bruin T W, van der Heide D
Acta Endocrinol (Copenh). 1982 Apr;99(4):522-9. doi: 10.1530/acta.0.0990522.
The influence of pH, salts, temperature and time of incubation on the binding of [125I]thyrotrophin to its human thyroid receptor was studied. Optimal binding was achieved at pH 7.0--7.6. Pre-incubation of membrane receptors at pH 6.0 resulted in a 60% loss of binding. There was dynamic equilibrium, as demonstrated by kinetic experiments. Scatchard analysis yielded non-linear plots with apparent affinity constants of 1.9 x 10(9) M-1 and 3.7 x 10(7) M-1. The concentration of [125I]thyrotrophin used in the experiments (1.2--2.2 pM) approximated normal human serum thyrotrophin concentration.
研究了pH值、盐类、温度及孵育时间对[125I]促甲状腺素与其人甲状腺受体结合的影响。在pH 7.0 - 7.6时可实现最佳结合。在pH 6.0条件下对膜受体进行预孵育会导致结合能力丧失60%。动力学实验表明存在动态平衡。Scatchard分析得到非线性图，表观亲和常数分别为1.9×10⁹ M⁻¹和3.7×10⁷ M⁻¹。实验中使用的[125I]促甲状腺素浓度（1.2 - 2.2 pM）接近正常人血清促甲状腺素浓度。
