Nadler P I, Miller G G, Sachs D H, Hodes R J
Eur J Immunol. 1982 Feb;12(2):113-20. doi: 10.1002/eji.1830120204.
The expression and functional significance of idiotypic determinants on antigen-specific helper T (Th) cell populations for responses to Staphylococcal nuclease (Nase) were evaluated in an in vitro antibody response system. Trinitrophenyl (TNP)- specific plaque-forming cell responses to TNP-conjugates of Nase (TNP-Nase) were shown to require the cooperation of Nase-primed Th cells as well as unprimed B and accessory cells. The expression on these antigen-primed Th cells of idiotypic determinants cross-reactive with those on anti-Nase antibodies was demonstrated by the specific elimination of Th cells for TNP-Nase by treatment with affinity-purified anti-idiotypic antibodies plus complement. The susceptibility of Nase-primed Th cells to elimination by such treatment was specific in that anti-idiotypic antibodies affected Th cells only from strains normally expressing the same (or a cross-reactive) idiotype on anti-Nase antibodies. A functional role of the idiotypes expressed on Nase-primed Th cells was suggested by the fact that anti-idiotypic antibody present throughout the period of culture, in the absence of complement, suppressed responses to TNP-Nase in an antigen- and strain-specific manner. It was further shown, by cell mixing experiments, that this inhibition appeared to occur at the level of the Th cells and was not dependent on the strain of origin of the B cells. Thus, antigen-specific Nase-primed Th cells express strain-specific idiotypic determinants cross-reactive with, or identical to, those of anti-Nase antibodies. These cell surface idiotypic determinants appear to be functionally involved in the activity of Th cells for the induction of antibody responses to TNP-Nase in vitro.
在体外抗体应答系统中，评估了抗原特异性辅助性T（Th）细胞群体上独特型决定簇对葡萄球菌核酸酶（Nase）应答的表达及功能意义。针对Nase的三硝基苯基（TNP）缀合物（TNP-Nase）的TNP特异性空斑形成细胞应答显示，需要Nase致敏的Th细胞以及未致敏的B细胞和辅助细胞的协同作用。用亲和纯化的抗独特型抗体加补体处理，可特异性清除针对TNP-Nase的Th细胞，从而证明这些抗原致敏的Th细胞上存在与抗Nase抗体上的独特型决定簇交叉反应的独特型决定簇。Nase致敏的Th细胞对这种处理的清除敏感性具有特异性，即抗独特型抗体仅影响来自正常在抗Nase抗体上表达相同（或交叉反应）独特型的菌株的Th细胞。Nase致敏的Th细胞上表达的独特型具有功能作用，这一事实表明，在培养期间全程存在的抗独特型抗体，在无补体的情况下，以抗原和菌株特异性方式抑制对TNP-Nase的应答。细胞混合实验进一步表明，这种抑制似乎发生在Th细胞水平，且不依赖于B细胞的来源菌株。因此，抗原特异性Nase致敏的Th细胞表达与抗Nase抗体的独特型决定簇交叉反应或相同的菌株特异性独特型决定簇。这些细胞表面独特型决定簇似乎在体外Th细胞诱导对TNP-Nase抗体应答的活性中发挥功能作用。
