Herpes simplex vaccine using cell membrane associated antigen in an animal model.

Vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of Herpes simplex virus type 2 (HSV-2) infected Vero cells with dithiothreitol and formaldehyde. These preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with HSV-2.