Kamatani N, Carson D A
Int Arch Allergy Appl Immunol. 1982;68(1):84-9. doi: 10.1159/000233071.
In an effort to find exploitable metabolic differences between human T and B lymphoblasts, we have compared the ability of lymphocytes of varying phenotype to grow in cystine-deficient medium. Only 6 of 12 human lymphoblastoid cell lines tested were able to utilize homocysteine thiolactone or cystathionine in place of cystine for growth. This difference in growth requirements was unrelated to the rate of cell division, the presence of Epstein-Barr viral genetic material, or whether or not the cell lines derived from benign or malignant tissues. Rather, all B lymphoblastoid cell lines grew in homocysteine thiolactone- or cystathionine-containing medium, while the T and non-T, non-B lymphoblastoid cell lines did not. Normal human peripheral blood T and B lymphoblasts did not respond to mitogens in the homocysteine thiolactone or cystathionine medium, but developed the ability to utilize these cysteine precursors after stimulation with concanavalin A, protein A, or Epstein-Barr virus. The differences in cysteine requirements among T and B cell lines may reflect a fundamental difference in de novo protein-synthesizing capacity of the two cell types.
为了找出人类T淋巴细胞和成淋巴细胞之间可利用的代谢差异，我们比较了不同表型淋巴细胞在缺乏胱氨酸的培养基中生长的能力。在测试的12个人类淋巴母细胞系中，只有6个能够利用高半胱氨酸硫内酯或胱硫醚代替胱氨酸进行生长。这种生长需求的差异与细胞分裂速率、EB病毒遗传物质的存在与否，或者细胞系来源于良性还是恶性组织无关。相反，所有B淋巴母细胞系都能在含有高半胱氨酸硫内酯或胱硫醚的培养基中生长，而T淋巴细胞系以及非T、非B淋巴母细胞系则不能。正常人类外周血T淋巴细胞和成淋巴细胞在高半胱氨酸硫内酯或胱硫醚培养基中对有丝分裂原无反应，但在用刀豆球蛋白A、蛋白A或EB病毒刺激后，会产生利用这些半胱氨酸前体的能力。T淋巴细胞系和成淋巴细胞系之间半胱氨酸需求的差异可能反映了这两种细胞类型在从头合成蛋白质能力上的根本差异。
