Matsuno S, Nakajima K
J Virol. 1982 Feb;41(2):710-4. doi: 10.1128/JVI.41.2.710-714.1982.
Single-stranded RNA (ssRNA) was transcribed in vitro from inner-shell particles of human rotavirus strain Wa (HRV-Wa) and a bovine rotavirus (neonatal calf diarrhea virus [NCDV]) by virion-associated RNA polymerase activity. The ssRNA product consisted of 11 RNA segments which were separated by polyacrylamide gel electrophoresis. In vitro-transcribed 32P-labeled ssRNA was used to study the genetic relatedness between rotaviruses by annealing with genomic double-stranded RNA (dsRNA) of homologous or heterologous rotavirus. All segments of HRV-Wa ssRNA were hybridized with dsRNA of HRV TK80, collected from the feces of a gastroenteritis patient, at the level of 88 to 100% of the homologous reaction. On the other hand, no segments of ssRNA from HRV-Wa hybridized with dsRNA of NCDV or simian rotavirus (simian agent 11). Similarly, ssRNA from NCDV did not hybridize with dsRNA of HRV-Wa, but hybridized with dsRNA of simian agent 11 at the level of 30% of the homologous value.
通过病毒体相关的RNA聚合酶活性，从人轮状病毒Wa株（HRV-Wa）和牛轮状病毒（新生小牛腹泻病毒 [NCDV]）的内壳颗粒体外转录单链RNA（ssRNA）。ssRNA产物由11个RNA片段组成，通过聚丙烯酰胺凝胶电泳分离。体外转录的32P标记的ssRNA用于通过与同源或异源轮状病毒的基因组双链RNA（dsRNA）退火来研究轮状病毒之间的遗传相关性。HRV-Wa ssRNA的所有片段与从一名肠胃炎患者粪便中收集的HRV TK80的dsRNA杂交，同源反应水平为88%至100%。另一方面，HRV-Wa的ssRNA片段与NCDV或猿猴轮状病毒（猿猴因子11）的dsRNA均未杂交。同样，NCDV的ssRNA与HRV-Wa的dsRNA未杂交，但与猿猴因子11的dsRNA杂交，杂交水平为同源值的30%。
