猿猴轮状病毒与牛轮状病毒之间重组体的分离。

Isolation of a recombinant between simian and bovine rotaviruses.

作者信息

Matsuno S, Hasegawa A, Kalica A R, Kono R

出版信息

J Gen Virol. 1980 May;48(1):253-6. doi: 10.1099/0022-1317-48-1-253.

Abstract

A recombinant between simian rotavirus, simian agent 11 (SA-11) and bovine rotavirus, neonatal calf diarrhoea virus (NCDV), was obtained by mixed infection of MA-104 cells with NCDV and u.v.-irradiated SA-11 virus, and isolation of a plaque formed in the presence of anti-NCDV serum. The genome of the recombinant contained dsRNA segments 4, 5 and 10 derived from SA-11 virus and segments 1, 2, 3, 6 and 11 derived from NCDV, and segments 7, 8 and 9 of undetermined origin. Polypeptides VP4, VP5, VP7a, VP7b, NCVP1 and MCVP3 were derived from SA-11 virus and polypeptides VP1, VP2, VP3, VP6, VP8, NCVP2a and NCVP2b from NCDV. Haemagglutination of the recombinant was inhibited and its infectivity neutralized by the antiserum against SA-11 virus but not by anti-NCDV serum.

摘要

通过用新生小牛腹泻病毒（NCDV）和紫外线照射的猴轮状病毒猿猴因子11（SA - 11）混合感染MA - 104细胞，并在抗NCDV血清存在的情况下分离形成的噬斑，获得了一种猴轮状病毒SA - 11和牛轮状病毒新生小牛腹泻病毒（NCDV）之间的重组体。该重组体的基因组包含源自SA - 11病毒的双链RNA片段4、5和10，以及源自NCDV的片段1、2、3、6和11，还有来源未确定的片段7、8和9。多肽VP4、VP5、VP7a、VP7b、NCVP1和MCVP3源自SA - 11病毒，而多肽VP1、VP2、VP3、VP6、VP8、NCVP2a和NCVP2b源自NCDV。该重组体的血凝反应被抗SA - 11病毒血清抑制，其感染性被该血清中和，但不被抗NCDV血清抑制。