Consolo S, Ladinsky H, Forloni G L, Grombi P
Life Sci. 1982 Mar 29;30(13):1113-20. doi: 10.1016/0024-3205(82)90532-x.
Electrolytic lesion of the ascending serotonergic fibers in the median raphe nucleus or in both the median raphe nucleus and dorsal raphe nucleus caused after 18 days more than 80% depletion of serotonin in the hippocampus and frontal cortex, respectively, without affecting norepinephrine and acetylcholine contents. alpha 1-Adrenoceptor binding of (3H) WB-4104 was increased in the hippocampus but not in the frontal cortex. Scatchard analysis revealed that the increase in (3H) WB-4101 binding in the lesioned hippocampus was the result of an elevated density of alpha 1-adrenergic receptors of about 65%. This phenomenon began 8 days postlesion and persisted for at least 90 days postlesion. Similar qualitative and quantitative results were obtained following chemical lesion of the serotonergic cells of origin in the median raphe nucleus with 5,7-dihydroxytryptamine. Selectivity of the phenomenon was further demonstrated as or beta-adrenoceptor binding with (3H) dihydroalprenolol and cholinergic muscarinic receptor binding with (3H) dexetimide were not significantly affected in the hippocampus. By comparison, when norepinephrine in the hippocampus was depleted by more than 90% by bilateral lesion of the ascending noradrenergic fibers with 6-hydroxydopamine (18 days), the alpha 1-adrenoceptor number was significantly increased by only about 20% while the beta-adrenoceptor number was enhance by 40%. The area-selective increase in alpha 1-adrenoceptor number in the hippocampus in the presence of unchanged norepinephrine content and in the absence of serotonin probably signifies that serotonin actively participates in the modulation of the noradrenergic receptor population.
在中缝核或中缝核与背缝核中对5-羟色胺能上行纤维进行电解损伤后，18天后海马和额叶皮质中的5-羟色胺分别耗竭超过80%，而不影响去甲肾上腺素和乙酰胆碱的含量。海马中(3H) WB - 4104的α1-肾上腺素能受体结合增加，但额叶皮质中未增加。Scatchard分析显示，损伤海马中(3H) WB - 4101结合的增加是由于α1-肾上腺素能受体密度升高约65%所致。这种现象在损伤后8天开始，并在损伤后至少持续90天。在用5,7-二羟基色胺对中缝核中5-羟色胺能起源细胞进行化学损伤后，获得了类似的定性和定量结果。该现象的选择性进一步得到证明，因为海马中与(3H)二氢阿普洛尔的β-肾上腺素能受体结合以及与(3H)右旋苯乙胺的胆碱能毒蕈碱受体结合均未受到显著影响。相比之下，当用6-羟基多巴胺双侧损伤上行去甲肾上腺素能纤维（18天）使海马中的去甲肾上腺素耗竭超过90%时，α1-肾上腺素能受体数量仅显著增加约20%，而β-肾上腺素能受体数量增加40%。在去甲肾上腺素含量不变且无5-羟色胺的情况下，海马中α1-肾上腺素能受体数量的区域选择性增加可能表明5-羟色胺积极参与去甲肾上腺素能受体群体的调节。
