Thode J, Børresen T, Beck K, Madsen S N
Acta Pharmacol Toxicol (Copenh). 1981 Oct;49(4):285-9. doi: 10.1111/j.1600-0773.1981.tb00907.x.
The effect of furosemide 8 X 10(-4) mol/l an 8 X 10(-5) mol/l on parathyroid hormone stimulated adenylate cyclase was studied in renal tissue slices from guinea pigs. Furosemide caused a dose-dependent inhibition of the effect of parathyroid hormone on production of cyclic AMP, without having any significant effect on the basal cyclic AMP production. Furosemide in similar concentrations did not inhibit the stimulatory effect of thyrotrophin and fluoride in human thyroid homogenates suggesting that furosemide is not an universal inhibitor of adenylate cyclase and that the inhibition is not caused by a direct action of furosemide on the adenylate cyclase enzyme. Furosemide did not interfere with binding of cyclic AMP to cyclic AMP binding protein kinase from rabbit muscle. The results indicate that furosemide exerts an inhibitory influence either upon binding of parathyroid hormone to renal receptors or upon transmission of impulse from receptor to adenylate cyclase. The inhibitory influence of furosemide on parathyroid hormone action in kidney could explain the value of furosemide in the acute treatment of hypercalcaemia, but also suggest that chronic treatment with furosemide might interfere with normal calcium metabolism.
在豚鼠肾组织切片中研究了8×10⁻⁴mol/l和8×10⁻⁵mol/l速尿对甲状旁腺激素刺激的腺苷酸环化酶的作用。速尿对甲状旁腺激素刺激环磷酸腺苷（cAMP）生成的作用产生剂量依赖性抑制，而对基础cAMP生成无显著影响。相似浓度的速尿在人甲状腺匀浆中不抑制促甲状腺激素和氟化物的刺激作用，这表明速尿不是腺苷酸环化酶的通用抑制剂，且这种抑制不是速尿直接作用于腺苷酸环化酶所致。速尿不干扰环磷酸腺苷与兔肌肉中环磷酸腺苷结合蛋白激酶的结合。结果表明，速尿要么对甲状旁腺激素与肾受体的结合产生抑制作用，要么对从受体到腺苷酸环化酶的冲动传递产生抑制作用。速尿对肾脏中甲状旁腺激素作用的抑制影响可以解释速尿在高钙血症急性治疗中的价值，但也提示速尿的长期治疗可能会干扰正常的钙代谢。
