Pace C S, Sachs G
Am J Physiol. 1982 May;242(5):C382-7. doi: 10.1152/ajpcell.1982.242.5.C382.
The weak base acridine orange (AO) has been shown to be accumulated by the insulin-containing secretory granules of cultured beta-cells in response to high glucose. Various lines of evidence indicate that this accumulation is due to a pH gradient. Thus ionophores such as monensin and nigericin abolish the glucose-induced accumulation, and a high concentration of the weak base, benzylamine, results in swelling of the granules. In the absence of glucose, ATP addition to digitonin-permeabilized cells also results in dye uptake. These data also suggest that a primary or secondary active accumulation mechanism for hydrogen ions exists across the granule membrane.
弱碱吖啶橙（AO）已被证明可被培养的β细胞中含胰岛素的分泌颗粒积累，以响应高葡萄糖。各种证据表明，这种积累是由于pH梯度所致。因此，离子载体如莫能菌素和尼日利亚菌素可消除葡萄糖诱导的积累，高浓度的弱碱苄胺会导致颗粒肿胀。在无葡萄糖的情况下，向洋地黄皂苷通透的细胞中添加ATP也会导致染料摄取。这些数据还表明，在颗粒膜上存在氢离子的初级或次级主动积累机制。
