Kitagawa S, Seki H, Kametani F, Sakurai H
Chem Biol Interact. 1982 Jul 1;40(3):265-74. doi: 10.1016/0009-2797(82)90150-8.
Hexavalent chromium (Cr(VI)) anion gradually penetrated into bovine erythrocytes and bound with cytoplasmic components. Its penetration was strongly inhibited by the NH2-reactive agent, 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonic acid (SITS) and the SH-reactive agent, N-ethylmaleimide (NEM). Gel filtration showed that the intracellular component that bound to chromium was hemoglobin. The binding affinity of Cr(VI) to hemoglobin in the absence of glutathione in vitro was found to be much less than in intact erythrocytes. However, in the presence of glutathione, the binding affinity of Cr(VI) to hemoglobin became much higher. This indicates that reduction of hemoglobin or Cr(VI) by glutathione is involved in the binding. Cr(VI) interacted only weakly with the membrane and did not cause hemolysis of bovine erythrocytes, unlike heavy metals such as Hg2+.
六价铬（Cr(VI)）阴离子逐渐渗透到牛红细胞中，并与细胞质成分结合。其渗透受到氨基反应剂4-乙酰氨基-4'-异硫氰基芪-2,2'-二磺酸（SITS）和巯基反应剂N-乙基马来酰亚胺（NEM）的强烈抑制。凝胶过滤显示，与铬结合的细胞内成分是血红蛋白。发现在体外无谷胱甘肽的情况下，Cr(VI)与血红蛋白的结合亲和力远低于完整红细胞中的情况。然而，在有谷胱甘肽存在的情况下，Cr(VI)与血红蛋白的结合亲和力变得更高。这表明谷胱甘肽对血红蛋白或Cr(VI)的还原参与了这种结合。与Hg2+等重金属不同，Cr(VI)与细胞膜的相互作用较弱，不会导致牛红细胞溶血。
