干酪乳杆菌二氢叶酸还原酶基因的分子克隆
Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei.
作者信息
Davies R W, Gronenborn A M
出版信息
Gene. 1982 Feb;17(2):229-33. doi: 10.1016/0378-1119(82)90077-4.
PMID:6282715
Abstract
The Lactobacillus casei gene for dihydrofolate reductase has been cloned in Escherichia coli using the multicopy vector pBR322. A restriction map of the cloned DNA has been prepared. The cloned DNA directs the synthesis of L. casei dihydrofolate reductase in E. coli and confers trimethoprim and methotrexate resistance.
摘要
干酪乳杆菌二氢叶酸还原酶基因已利用多拷贝载体pBR322在大肠杆菌中克隆出来。已制备出克隆DNA的限制性图谱。克隆的DNA在大肠杆菌中指导干酪乳杆菌二氢叶酸还原酶的合成，并赋予对甲氧苄氨嘧啶和氨甲蝶呤的抗性。
Zotero 插件