枯草芽孢杆菌DNA促旋酶：亚基的纯化及超螺旋活性的重组
Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.
作者信息
Orr E, Staudenbauer W L
出版信息
J Bacteriol. 1982 Jul;151(1):524-7. doi: 10.1128/jb.151.1.524-527.1982.
Abstract
DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.
摘要
来自枯草芽孢杆菌168的DNA促旋酶通过新生霉素-琼脂糖亲和层析法进行纯化，结果显示它由两个亚基组成，分别为A和B，分子量分别为100,000和85,000。含有对新生霉素敏感的ATP酶活性的B亚基能够补充大肠杆菌的gyrA蛋白。未检测到A亚基与大肠杆菌gyrB蛋白之间的互补作用。
Zotero 插件