编码DNA促旋酶A和B蛋白的金黄色葡萄球菌gyrB-gyrA基因座的核苷酸序列。
Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins.
作者信息
Margerrison E E, Hopewell R, Fisher L M
机构信息
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, Cranmer Terrace, London, United Kingdom.
出版信息
J Bacteriol. 1992 Mar;174(5):1596-603. doi: 10.1128/jb.174.5.1596-1603.1992.
Abstract
We have determined the nucleotide sequence of a 5.3-kb segment of the Staphylococcus aureus chromosome that includes the gyrA and gyrB genes coding for both subunits of DNA gyrase, the enzyme that catalyzes ATP-dependent DNA supercoiling. The gene order at this locus, dnaA-dnaN-recF-gyrB-gyrA, is similar to that found in the Bacillus subtilis replication origin region. S. aureus recF, gyrB, and gyrA genes are closely spaced, occupy the same reading frame, and may be coordinately expressed. The S. aureus gyrB and gyrA genes encode 640- and 889-residue proteins, respectively, that share strong homology with other bacterial gyrase subunits, notably those from B. subtilis. These results are discussed in regard to the mechanism of DNA gyrase and its role as a target for the 4-quinolones and other antistaphylococcal agents.
摘要
我们已经确定了金黄色葡萄球菌染色体上一段5.3kb片段的核苷酸序列，该片段包含编码DNA促旋酶两个亚基的gyrA和gyrB基因，DNA促旋酶是一种催化ATP依赖性DNA超螺旋化的酶。该位点的基因顺序，即dnaA-dnaN-recF-gyrB-gyrA，与在枯草芽孢杆菌复制起始区域中发现的顺序相似。金黄色葡萄球菌的recF、gyrB和gyrA基因紧密排列，位于同一阅读框内，可能协同表达。金黄色葡萄球菌的gyrB和gyrA基因分别编码640个和889个氨基酸的蛋白质，它们与其他细菌的促旋酶亚基具有很强的同源性，尤其是来自枯草芽孢杆菌的那些亚基。我们将结合DNA促旋酶的作用机制及其作为4-喹诺酮类药物和其他抗葡萄球菌药物靶点的作用来讨论这些结果。
相似文献
1
Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins.编码DNA促旋酶A和B蛋白的金黄色葡萄球菌gyrB-gyrA基因座的核苷酸序列。
J Bacteriol. 1992 Mar;174(5):1596-603. doi: 10.1128/jb.174.5.1596-1603.1992.
2
Cloning, sequencing, and expression of the DNA gyrase genes from Staphylococcus aureus.金黄色葡萄球菌DNA回旋酶基因的克隆、测序及表达
J Bacteriol. 1993 Jun;175(11):3269-77. doi: 10.1128/jb.175.11.3269-3277.1993.
3
DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance.金黄色葡萄球菌gyrA和gyrB基因的DNA克隆与组织：促旋酶蛋白间的高度同源性及其对4-喹诺酮作用和耐药性的影响
J Bacteriol. 1990 Jun;172(6):3481-4. doi: 10.1128/jb.172.6.3481-3484.1990.
4
Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus.金黄色葡萄球菌DNA旋转酶gyrA和gyrB基因中的喹诺酮抗性突变。
Antimicrob Agents Chemother. 1994 Sep;38(9):2014-23. doi: 10.1128/AAC.38.9.2014.
5
Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in fluoroquinolone resistance.肺炎链球菌编码DNA拓扑异构酶IV的parC和parE基因的克隆与特性分析：在氟喹诺酮耐药性中的作用
J Bacteriol. 1996 Jul;178(14):4060-9. doi: 10.1128/jb.178.14.4060-4069.1996.
6
Nucleotide sequence of the recF gene cluster from Staphylococcus aureus and complementation analysis in Bacillus subtilis recF mutants.金黄色葡萄球菌recF基因簇的核苷酸序列及在枯草芽孢杆菌recF突变体中的互补分析。
Mol Gen Genet. 1995 Mar 20;246(6):680-6. doi: 10.1007/BF00290713.
7
Discoordinate gene expression of gyrA and gyrB in response to DNA gyrase inhibition in Escherichia coli.大肠杆菌中gyrA和gyrB基因表达失调以响应DNA回旋酶抑制作用。
J Basic Microbiol. 1997;37(1):53-69. doi: 10.1002/jobm.3620370109.
8
Mycobacterium smegmatis DNA gyrase: cloning and overexpression in Escherichia coli.耻垢分枝杆菌DNA促旋酶：在大肠杆菌中的克隆与过表达
Microbiology (Reading). 1995 Dec;141 ( Pt 12):3029-37. doi: 10.1099/13500872-141-12-3029.
9
Differential behaviors of Staphylococcus aureus and Escherichia coli type II DNA topoisomerases.金黄色葡萄球菌和大肠杆菌II型DNA拓扑异构酶的差异行为
Antimicrob Agents Chemother. 1996 Dec;40(12):2714-20. doi: 10.1128/AAC.40.12.2714.
10
Cloning and sequencing of the replication origin (oriC) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids.柑橘螺原体染色体复制起点（oriC）的克隆与测序及自主复制人工质粒的构建。
Curr Microbiol. 1994 Jul;29(1):23-9. doi: 10.1007/BF01570187.
引用本文的文献
1
Machine learning and network analysis with focus on the biofilm in .专注于生物膜的机器学习与网络分析。 （注：原文最后in后面缺少具体内容，译文按补充完整后的意思翻译）
Comput Struct Biotechnol J. 2024 Nov 10;23:4148-4160. doi: 10.1016/j.csbj.2024.11.011. eCollection 2024 Dec.
2
Generation and Repair of Postreplication Gaps in Escherichia coli.大肠杆菌复制后缺口的产生和修复。
Microbiol Mol Biol Rev. 2023 Jun 28;87(2):e0007822. doi: 10.1128/mmbr.00078-22. Epub 2023 May 22.
3
The anti-methicillin-resistant Staphylococcus aureus quinolone WCK 771 has potent activity against sequentially selected mutants, has a narrow mutant selection window against quinolone-resistant Staphylococcus aureus, and preferentially targets DNA gyrase.抗耐甲氧西林金黄色葡萄球菌喹诺酮类药物WCK 771对序贯选择的突变体具有强大活性，对耐喹诺酮金黄色葡萄球菌的突变选择窗较窄，且优先靶向DNA旋转酶。
Antimicrob Agents Chemother. 2006 Nov;50(11):3568-79. doi: 10.1128/AAC.00641-06. Epub 2006 Aug 28.
4
Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania.从宾夕法尼亚州一名患者身上分离出的耐万古霉素金黄色葡萄球菌。
Antimicrob Agents Chemother. 2004 Jan;48(1):275-80. doi: 10.1128/AAC.48.1.275-280.2004.
5
In vitro antibacterial potency and spectrum of ABT-492, a new fluoroquinolone.新型氟喹诺酮类药物ABT-492的体外抗菌效力及抗菌谱
Antimicrob Agents Chemother. 2003 Oct;47(10):3260-9. doi: 10.1128/AAC.47.10.3260-3269.2003.
6
Small-colony mutants of Staphylococcus aureus allow selection of gyrase-mediated resistance to dual-target fluoroquinolones.金黄色葡萄球菌的小菌落突变体有助于筛选出由回旋酶介导的对双靶点氟喹诺酮类药物的耐药性。
Antimicrob Agents Chemother. 2002 Aug;46(8):2498-506. doi: 10.1128/AAC.46.8.2498-2506.2002.
7
Mechanisms and frequency of resistance to gatifloxacin in comparison to AM-1121 and ciprofloxacin in Staphylococcus aureus.金黄色葡萄球菌中加替沙星与AM-1121及环丙沙星相比的耐药机制和耐药频率
Antimicrob Agents Chemother. 2001 Oct;45(10):2755-64. doi: 10.1128/AAC.45.10.2755-2764.2001.
8
Cloning and nucleotide sequence of the DNA gyrase (gyrA) gene from Mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin.人型支原体DNA促旋酶（gyrA）基因的克隆、核苷酸序列分析及体外筛选的曲伐沙星耐药突变株的特性研究
Antimicrob Agents Chemother. 2000 Oct;44(10):2719-27. doi: 10.1128/AAC.44.10.2719-2727.2000.
9
Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.大肠杆菌和噬菌体λ中DNA损伤的重组修复
Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents. doi: 10.1128/MMBR.63.4.751-813.1999.
10
Activities of trovafloxacin compared with those of other fluoroquinolones against purified topoisomerases and gyrA and grlA mutants of Staphylococcus aureus.曲伐沙星与其他氟喹诺酮类药物对金黄色葡萄球菌纯化的拓扑异构酶以及gyrA和grlA突变体的活性比较。
Antimicrob Agents Chemother. 1999 Aug;43(8):1845-55. doi: 10.1128/AAC.43.8.1845.
本文引用的文献
1
DNA gyrase action involves the introduction of transient double-strand breaks into DNA.DNA 回旋酶的作用涉及在 DNA 中引入瞬时双链断裂。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1847-51. doi: 10.1073/pnas.77.4.1847.
2
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
3
Mapping the active site tyrosine of Escherichia coli DNA gyrase.绘制大肠杆菌DNA促旋酶的活性位点酪氨酸图谱。
J Biol Chem. 1987 Apr 15;262(11):5339-44.
4
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.大肠杆菌gyrB基因的DNA序列：一种新测序策略的应用
Nucleic Acids Res. 1987 Jan 26;15(2):771-84. doi: 10.1093/nar/15.2.771.
5
Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli.大肠杆菌gyrB基因的萘啶酸抗性突变
Mol Gen Genet. 1986 Sep;204(3):367-73. doi: 10.1007/BF00331012.
6
DNA gyrase complex with DNA: determinants for site-specific DNA breakage.与DNA结合的DNA促旋酶复合物：位点特异性DNA断裂的决定因素
EMBO J. 1986 Jun;5(6):1411-8. doi: 10.1002/j.1460-2075.1986.tb04375.x.
7
Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli.枯草芽孢杆菌和大肠杆菌染色体复制起始区域中基因及其组织的保守性。
EMBO J. 1985 Dec 1;4(12):3345-50. doi: 10.1002/j.1460-2075.1985.tb04087.x.
8
DNA topoisomerases.DNA拓扑异构酶
Annu Rev Biochem. 1985;54:665-97. doi: 10.1146/annurev.bi.54.070185.003313.
9
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.枯草芽孢杆菌染色体复制起点区域的结构与功能。III. 起点区域约10,000个碱基对的核苷酸序列。
Nucleic Acids Res. 1985 Apr 11;13(7):2251-65. doi: 10.1093/nar/13.7.2251.
10
Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis.枯草芽孢杆菌克隆的gyrA和gyrB基因的遗传与物理组织
J Bacteriol. 1985 Apr;162(1):78-84. doi: 10.1128/jb.162.1.78-84.1985.
Zotero 插件