肝高尔基体组分可分解为膜亚组分和内容物亚组分。
Hepatic Golgi fractions resolved into membrane and content subfractions.
作者信息
Howell K E, Palade G E
出版信息
J Cell Biol. 1982 Mar;92(3):822-32. doi: 10.1083/jcb.92.3.822.
Abstract
Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fractions was assessed by electron microscopy and biochemical assays. The membrane subfraction retained 15% of content proteins and lipids, and these could not be removed by various washing procedures. The content subfraction was contaminated by both membrane fragments and vesicles and accounted for 5 to 10% of the membrane enzyme activities of the original Golgi fraction. The lipid compositions of the subfractions was determined, and the phospholipids of both membrane and content were found to be uniformly labeled with [33P]phosphate administered in vivo.
摘要
从大鼠肝脏匀浆中分离出的高尔基体组分，通过用100 mM碳酸钠（pH 11.3）处理，已被分解为膜亚组分和内容物亚组分。该程序允许大量提取内容物蛋白质和脂蛋白，推测这是因为它导致高尔基体膜发生改变，从而使封闭囊泡的重新形成减到最少。通过电子显微镜和生化分析评估了各组分的污染类型和程度。膜亚组分保留了15%的内容物蛋白质和脂质，并且这些物质不能通过各种洗涤程序去除。内容物亚组分被膜碎片和囊泡污染，占原始高尔基体组分膜酶活性的5%至10%。测定了亚组分的脂质组成，发现膜和内容物的磷脂在用体内给予的[33P]磷酸盐标记后是均匀标记的。
相似文献
1
Hepatic Golgi fractions resolved into membrane and content subfractions.肝高尔基体组分可分解为膜亚组分和内容物亚组分。
J Cell Biol. 1982 Mar;92(3):822-32. doi: 10.1083/jcb.92.3.822.
2
Heterogeneity of lipoprotein particles in hepatic Golgi fractions.肝高尔基体组分中脂蛋白颗粒的异质性
J Cell Biol. 1982 Mar;92(3):833-45. doi: 10.1083/jcb.92.3.833.
3
The lipids of the Golgi apparatus subfractions from rat liver.来自大鼠肝脏的高尔基体亚组分的脂质。
Biochim Biophys Acta. 1979 May 25;573(2):301-7. doi: 10.1016/0005-2760(79)90063-8.
4
The lipid composition of plasma membrane subfractions originating from the three major functional domains of the rat hepatocyte cell surface.源自大鼠肝细胞表面三个主要功能域的质膜亚组分的脂质组成。
Biochim Biophys Acta. 1976 Dec 14;455(3):655-64. doi: 10.1016/0005-2736(76)90039-0.
5
The transverse distribution of phospholipids in the membranes of Golgi subfractions of rat hepatocytes.大鼠肝细胞高尔基体亚组分膜中磷脂的横向分布。
Biochem J. 1984 Apr 1;219(1):261-72. doi: 10.1042/bj2190261.
6
7
Characterization of intracisternal and membrane subfractions from sonically disrupted rough microsomal and Golgi-rich fractions of the rat liver.大鼠肝脏经超声破碎的粗面微粒体和富含高尔基体的组分中脑池内和膜亚组分的表征
Exp Cell Res. 1978 Mar 1;112(1):187-97. doi: 10.1016/0014-4827(78)90539-6.
8
9
Distribution of clathrin and spiny-coated vesicles on membranes within mature Golgi apparatus elements of mouse liver.网格蛋白和棘状被膜小泡在小鼠肝脏成熟高尔基体元件内的膜上的分布。
Eur J Cell Biol. 1982 Aug;28(1):130-8.
10
Phospholipids of Golgi subfractions determined with an enzymatic method.用酶法测定高尔基体亚组分的磷脂。
Exp Pathol. 1984;26(2):107-11. doi: 10.1016/s0232-1513(84)80077-8.
引用本文的文献
1
TANGO6 regulates cell proliferation via COPI vesicle-mediated RPB2 nuclear entry.TANGO6 通过 COPI 囊泡介导的 RPB2 核输入调节细胞增殖。
Nat Commun. 2024 Mar 15;15(1):2371. doi: 10.1038/s41467-024-46720-y.
2
Potential Role of Oral Rinses Targeting the Viral Lipid Envelope in SARS-CoV-2 Infection.针对 SARS-CoV-2 感染的病毒脂包膜的口腔冲洗在潜在作用。
Function (Oxf). 2020;1(1):zqaa002. doi: 10.1093/function/zqaa002. Epub 2020 Jun 5.
3
Deep interactome profiling of membrane proteins by co-interacting protein identification technology.通过共相互作用蛋白鉴定技术对膜蛋白进行深入的互作组学分析。
Nat Protoc. 2016 Dec;11(12):2515-2528. doi: 10.1038/nprot.2016.140. Epub 2016 Nov 17.
4
Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231.人乳腺癌细胞系MCF7和MDA-MB-231中高尔基体磷蛋白3的不同生化池
PLoS One. 2016 Apr 28;11(4):e0154719. doi: 10.1371/journal.pone.0154719. eCollection 2016.
5
SPACA7 is a novel male germ cell-specific protein localized to the sperm acrosome that is involved in fertilization in mice.SPACA7是一种新发现的雄性生殖细胞特异性蛋白，定位于精子顶体，参与小鼠的受精过程。
Biol Reprod. 2014 Jan 30;90(1):16. doi: 10.1095/biolreprod.113.111831. Print 2014 Jan.
6
Bioanalysis of eukaryotic organelles.真核细胞器的生物分析
Chem Rev. 2013 Apr 10;113(4):2733-811. doi: 10.1021/cr300354g.
7
Widespread Occurrence of Non-Enzymatic Deamidations of Asparagine Residues in Yersinia pestis Proteins Resulting from Alkaline pH Membrane Extraction Conditions.碱性pH膜提取条件导致鼠疫耶尔森菌蛋白质中天冬酰胺残基非酶促脱酰胺作用的广泛发生。
Open Proteomics J. 2008 Jan 1;1:106-115. doi: 10.2174/1875039700801010106.
8
Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglions.大鼠背根神经节质膜的富集与蛋白质组学分析
Proteome Sci. 2009 Nov 5;7:41. doi: 10.1186/1477-5956-7-41.
9
Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.载脂蛋白 M 的表达增加了由三磷酸腺苷结合盒转运体 A1 形成的新生前 β HDL 的大小。
J Lipid Res. 2010 Mar;51(3):514-24. doi: 10.1194/jlr.M002162. Epub 2009 Sep 18.
10
A helical membrane-binding domain targets the Toxoplasma ROP2 family to the parasitophorous vacuole.一个螺旋膜结合结构域将弓形虫 ROP2 家族靶向到寄生空泡。
Traffic. 2009 Oct;10(10):1458-70. doi: 10.1111/j.1600-0854.2009.00958.x. Epub 2009 Jun 22.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A FAST, SIMPLE AND RELIABLE METHOD FOR THE MICRODETERMINATION OF PHOSPHORUS IN BIOLOGICAL MATERIALS.一种用于微量测定生物材料中磷的快速、简单且可靠的方法。
Anal Biochem. 1964 Feb;7:218-24. doi: 10.1016/0003-2697(64)90231-3.
3
The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.多胺在噬菌体脱氧核糖核酸中和中的作用。
J Biol Chem. 1960 Mar;235:769-75.
4
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
5
Secretion granules of the rabbit parotid. Selective removal of secretory contaminants from granule membranes.兔腮腺的分泌颗粒。从颗粒膜中选择性去除分泌性污染物。
J Cell Biol. 1978 Feb;76(2):323-40. doi: 10.1083/jcb.76.2.323.
6
Distribution of terminal glycosyltransferases in hepatic Golgi fractions.末端糖基转移酶在肝高尔基体组分中的分布。
J Cell Biol. 1980 Jan;84(1):87-101. doi: 10.1083/jcb.84.1.87.
7
Heterogeneity of lipoprotein particles in hepatic Golgi fractions.肝高尔基体组分中脂蛋白颗粒的异质性
J Cell Biol. 1982 Mar;92(3):833-45. doi: 10.1083/jcb.92.3.833.
8
Lipid synthesis, intracellular transport, storage, and secretion. I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats.脂质合成、细胞内运输、储存及分泌。I. 禁食及乙醇处理大鼠注射氚标记棕榈酸酯或甘油后肝脏的电子显微镜放射自显影研究。
J Cell Biol. 1967 May;33(2):319-39. doi: 10.1083/jcb.33.2.319.
9
Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules.胰腺外分泌细胞中分泌蛋白的细胞内运输。II. 向浓缩泡和酶原颗粒的运输。
J Cell Biol. 1967 Aug;34(2):597-615. doi: 10.1083/jcb.34.2.597.
Zotero 插件