Osterhaus A D, Teppema J S, Wirahadiredja R M, van Steenis G
Lab Anim Sci. 1981 Dec;31(6):704-6.
Two independent outbreaks of ectromelia in mice occurred in The Netherlands. In both cases, the causative virus was isolated and identified as ectromelia virus on the basis of serology, demonstration of antigen by indirect immunofluorescence, negative contrast electron microscopy, morphology of lesions on chorioallantoic membranes of embryonated chicken eggs, and cytopathogenicity for mouse cells. Inoculation of the virus into the dermis of rabbits demonstrated a low virulence for this species.
在荷兰发生了两起小鼠感染脱脚病的独立疫情。在这两起疫情中，均分离出了致病病毒，并通过血清学、间接免疫荧光法检测抗原、负染电子显微镜观察、鸡胚绒毛尿囊膜上病变的形态学以及该病毒对小鼠细胞的细胞致病性，将其鉴定为脱脚病病毒。将该病毒接种到兔子的真皮内，结果表明该病毒对兔子的毒力较低。
