Endoh M, Nakase Y
Microbiol Immunol. 1982;26(2):119-27. doi: 10.1111/j.1348-0421.1982.tb00161.x.
The effects of sensitization with the histamine-sensitizing factor (HSF) of Bordetella pertussis as well as Bordetella vaccines on a pharmacologic response in rat heart preparations were determined. In normal rats the spontaneous beating of atria in vitro through the positive inotropic action produced by the addition of epinephrine was inhibited immediately by addition of acetylcholine, whereas in the B. pertussis vaccine-treated rats the exciting atria were scarcely inhibited by acetylcholine. Neither B. parapertussis nor B. bronchiseptica vaccines induced such an altered atrial response in rats. Of the B. pertussis cell components purified HSF induced the altered response at the minimal dose of 0.1 microgram per rat, and a dose of 1 microgram or more produced the maximal change. This altered atrial-inducing activity of HSF was inactivated by heating at 63 C for 30 min, and was neutralized by anti-HSF rabbit serum. The altered response rose quickly in 1 day after i.v. injection of 1 microgram of HSF, reached a plateau in 3 to 5 days, which lasted at least 14 days, and disappeared completely 56 days later. HSF failed to produce directly any functional damage to the beating atria in vitro, and to induce the altered response of the normal rat atria by incubation with as much as 10 microgram of HSF per bath (50 ml) for 4 hr. A trace stimulation was found in the normal rat atria as well as in perfused frog hearts, if HSF was given directly at a dose of 20 microgram per bath.
测定了百日咳博德特氏菌的组胺致敏因子（HSF）以及百日咳博德特氏菌疫苗致敏对大鼠心脏制剂药理反应的影响。在正常大鼠中，通过添加肾上腺素产生的正性肌力作用使体外心房的自发搏动立即被乙酰胆碱抑制，而在经百日咳博德特氏菌疫苗处理的大鼠中，兴奋的心房几乎不被乙酰胆碱抑制。副百日咳博德特氏菌和支气管败血博德特氏菌疫苗均未在大鼠中诱导出这种改变的心房反应。在百日咳博德特氏菌细胞成分中，纯化的HSF以每只大鼠0.1微克的最小剂量诱导出改变的反应，1微克或更高剂量产生最大变化。HSF的这种改变心房的活性在63℃加热30分钟后失活，并被抗HSF兔血清中和。静脉注射1微克HSF后1天内，改变的反应迅速上升，3至5天达到平台期，持续至少14天，56天后完全消失。HSF在体外未能对跳动的心房产生任何直接的功能损害，并且在每浴（50毫升）加入多达10微克HSF孵育4小时的情况下，未能诱导正常大鼠心房的改变反应。如果以每浴20微克的剂量直接给予HSF，则在正常大鼠心房以及灌注的蛙心中发现微量刺激。
