血蓝蛋白生物聚合物光谱探针衍生物的制备：别构相互作用对双核铜活性位点的影响
Preparation of a spectral probe derivative of the hemocyanin biopolymer: effects of allosteric interactions on the coupled binuclear copper active site.
作者信息
Hwang Y T, Solomon E I
出版信息
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2564-8. doi: 10.1073/pnas.79.8.2564.
Abstract
A series of derivatives for both the arthropod and mollusc hemocyanin biopolymers has been prepared; the derivatives contain a small fraction of electron paramagnetic resonance-detectable half-met [Cu(II) CU(I)] sites dispersed among the non-detectable oxy binuclear copper active sites. Upon deoxygenation, large changes in the electron paramagnetic resonance signal of these half-met spectral probe derivatives are observed, which are further adjusted by the heterotropic effectors Ca2+ and H+. The active site structural changes indicated that these spectral changes as the hemocyanins go from a relaxed to a tensed quaternary structure are then discussed.
摘要
已经制备了一系列节肢动物和软体动物血蓝蛋白生物聚合物的衍生物；这些衍生物含有一小部分可通过电子顺磁共振检测到的半金属[Cu(II) Cu(I)]位点，它们分散在不可检测的氧双核铜活性位点之中。脱氧后，观察到这些半金属光谱探针衍生物的电子顺磁共振信号发生了很大变化，这些变化会进一步受到异向效应物Ca2+和H+的调节。然后讨论了活性位点的结构变化，这些变化表明血蓝蛋白从松弛的四级结构转变为紧张的四级结构时的光谱变化。
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