Light A, Morgan E H
Scand J Haematol. 1982 Mar;28(3):205-14. doi: 10.1111/j.1600-0609.1982.tb00516.x.
The endocytosis of transferrin by rabbit reticulocytes was investigated by electron microscopy using transferrin labelled with colloidal gold. This complex was shown to bind and donate its iron to the cells in a manner comparable to native iron-transferrin. After incubation at 37 degrees C approximately 70% of the transferrin-gold particles were located within endocytotic vesicles. Treatment of the cells with pronase, EDTA, metabolic inhibitors and heating to 46 degrees C inhibited the endocytosis. The uptake of colloidal gold complexes of albumin and concanavalin A were compared with that of transferrin. Little endocytosis of these proteins was observed. It is concluded that colloidal gold is a suitable label for the investigation of transferrin endocytosis and that the endocytosis is specific for transferrin and sensitive to the action of reagents which damage membrane receptors, alter membrane function or impair cell metabolism.
利用胶体金标记的转铁蛋白，通过电子显微镜研究了兔网织红细胞对转铁蛋白的内吞作用。结果表明，这种复合物能够以与天然铁转铁蛋白类似的方式结合并向细胞输送铁。在37℃孵育后，约70%的转铁蛋白 - 金颗粒位于内吞小泡内。用链霉蛋白酶、乙二胺四乙酸（EDTA）、代谢抑制剂处理细胞以及加热至46℃均抑制了内吞作用。将白蛋白和伴刀豆球蛋白A的胶体金复合物的摄取与转铁蛋白的摄取进行了比较。几乎未观察到这些蛋白质的内吞作用。得出的结论是，胶体金是研究转铁蛋白内吞作用的合适标记物，并且内吞作用对转铁蛋白具有特异性，对破坏膜受体、改变膜功能或损害细胞代谢的试剂的作用敏感。
