Watmough D J
Acta Radiol Oncol. 1982;21(1):11-5. doi: 10.3109/02841868209133978.
Preliminary measurements showed that spectrophotometry of samples of tissue taken at operation from the female breast demonstrated no obvious correlation between transmission coefficient and pathology, except that thicker samples tended to transmit less light than thinner samples. However all the samples had absorption minima corresponding to the bands of oxyhaemoglobin. This simple fact led to the speculation that the density of blood in the tissues, (number of red cells per unit volume) was the factor giving rise to different colours of breast tissues and of breast pathology observed upon transillumination with white light in vivo. Examination by infrared colour photography and by spectrophotometry of transilluminated samples of blood diluted with normal saline confirms that the observed colour is dependent on red cell concentration.
初步测量表明，对女性乳房手术切除组织样本进行分光光度测定时，除了较厚的样本往往比较薄的样本透光性差外，透射系数与病理结果之间未显示出明显的相关性。然而，所有样本都有与氧合血红蛋白谱带相对应的吸收最小值。这一简单事实引发了一种推测，即组织中的血液密度（每单位体积红细胞数量）是导致在体内用白光透照时观察到的乳房组织和乳房病变呈现不同颜色的因素。通过红外彩色摄影以及对用生理盐水稀释的血液透照样本进行分光光度测定证实，观察到的颜色取决于红细胞浓度。
