Roos D, van Zwieten R, van Schaik M L, Hamers M N
Adv Exp Med Biol. 1982;141:323-34. doi: 10.1007/978-1-4684-8088-7_31.
The oxygen consumption, superoxide production and hydrogen peroxide generation was studied in human neutrophils phagocytosing zymosan particles. Application of sodium azide, as an inhibitor of catalase, and/or 1,3-bis(chloroethyl)-1-nitrosourea (BCNU), as an inhibitor of glutathione reductase, led to the conclusion that neutrophils convert about half of the oxygen consumed in the respiratory burst to hydrogen peroxide; the other half is used for formation of organic peroxides, disulfide bridges, etc. These products are rapidly degraded to water by catalase and/or the glutathione redox cycle. Reduction of exogenous cytochrome C accounted for only about 15% of the consumed oxygen. Neutrophil homogenates contain a badly damaged oxidase system, because oxygen consumption and hydrogen peroxide formation were only about one-tenth of that observed with whole cells. In contrast, cytochrome-C reduction was about three times as high as that found with intact cells. Probably, cytochrome C partly reconstitutes damaged oxidase systems, thus artificially increasing the oxidase activity. We conclude that cytochrome-C reduction is not a good parameter to characterize cell-free oxidase preparations.
在人类嗜中性粒细胞吞噬酵母聚糖颗粒的过程中，对其耗氧量、超氧化物生成量和过氧化氢生成量进行了研究。应用叠氮化钠（作为过氧化氢酶的抑制剂）和/或1,3-双（氯乙基）-1-亚硝基脲（BCNU，作为谷胱甘肽还原酶的抑制剂）后得出结论：嗜中性粒细胞在呼吸爆发过程中消耗的氧气约有一半转化为过氧化氢；另一半则用于形成有机过氧化物、二硫键等。这些产物通过过氧化氢酶和/或谷胱甘肽氧化还原循环迅速降解为水。外源性细胞色素C的还原仅占消耗氧气的约15%。嗜中性粒细胞匀浆含有严重受损的氧化酶系统，因为其耗氧量和过氧化氢生成量仅为全细胞观察值的约十分之一。相比之下，细胞色素C的还原量约为完整细胞的三倍。可能是细胞色素C部分重构了受损的氧化酶系统，从而人为地增加了氧化酶活性。我们得出结论，细胞色素C的还原不是表征无细胞氧化酶制剂的良好参数。
