Takeshige K, Matsumoto T, Nabi Z F, Minakami S
Adv Exp Med Biol. 1982;141:453-61. doi: 10.1007/978-1-4684-8088-7_43.
The relationship between the intracellular translocation of calcium from the storage pool and the oxidative metabolism was studied. An intracellular calcium-antagonist, TMB-8, inhibited the release of superoxide induced by a calcium ionophore A23187 and the inhibition was relieved by the addition of calcium ions. The release induced by cytochalasin D or by the ingestion of bacteria was similarly inhibited by TMB-8. The mobilization of intracellular divalent cations of leukocytes was monitored by a fluorescent probe, CTC. When the CTC-loaded cells were stimulated with cytochalasin D or E. coli, a fluorescence change ascribable to the release of calcium from the intracellular hydrophobic environment was observed. The dose-response curve of the fluorescence change and that of the superoxide release of th cells were very similar. TMB-8 inhibited both metabolic and fluorescence changes in parallel. The results support the hypothesis that an intracellular translocation of calcium is stimulated the oxidative metabolism of leukocytes.
研究了储存池内钙的细胞内转运与氧化代谢之间的关系。一种细胞内钙拮抗剂TMB - 8抑制了钙离子载体A23187诱导的超氧化物释放，加入钙离子后这种抑制作用得到缓解。细胞松弛素D或细菌摄入诱导的释放同样被TMB - 8抑制。用荧光探针CTC监测白细胞内二价阳离子的动员。当用细胞松弛素D或大肠杆菌刺激加载CTC的细胞时，观察到归因于细胞内疏水环境中钙释放的荧光变化。th细胞荧光变化的剂量反应曲线与超氧化物释放的剂量反应曲线非常相似。TMB - 8同时平行抑制代谢和荧光变化。结果支持这样的假设，即钙的细胞内转运刺激了白细胞的氧化代谢。
