尼日利亚活力充沛和窒息新生儿脐带血中溶酶体水解酶的变化。

Changes in lysosomal hydrolases from cord blood of vigorous and asphyxiated Nigerian newborn infants.

作者信息

Omene J A, Glew R H, Adamson I, Ihongbe J C

出版信息

Afr J Med Med Sci. 1980 Sep-Dec;9(3-4):81-3.

Abstract

The activities of lysosomal hydrolases, beta-glucuronidase and acid phosphatase, were assayed in cord sera from forty-seven non asphyxiated babies and eleven babies who were severely asphyxiated at birth. Significant changes of these enzymes were observed in cord serum. Compared to the control group, serum beta glucuronidase (determined at pH 4.5 using fluorogenic substrate, 4-methyl-umbelliferyl-B-D glucuronide) was 2.2 fold higher (P less than 0.01) in the asphyxiated babies. Similarly, serum acid phosphatase activity estimated by using 4-methyl-umbelliferyl-phosphate was 1.8 fold higher (P less than 0.01) in asphyxia neonatorum. The clinical relevance of this finding lies in its ultimate application, using the lysosomal hydrolase activity in scalp blood sample to evaluate objectively the foetal well-being.

摘要

对47名未窒息婴儿和11名出生时严重窒息婴儿的脐血血清中的溶酶体水解酶、β-葡萄糖醛酸酶和酸性磷酸酶的活性进行了测定。在脐血血清中观察到了这些酶的显著变化。与对照组相比，窒息婴儿血清β-葡萄糖醛酸酶（使用荧光底物4-甲基伞形酮基-β-D-葡萄糖醛酸在pH 4.5下测定）高出2.2倍（P<0.01）。同样，使用4-甲基伞形酮基磷酸酯估计的新生儿窒息血清酸性磷酸酶活性高出1.8倍（P<0.01）。这一发现的临床意义在于其最终应用，即利用头皮血样中的溶酶体水解酶活性来客观评估胎儿健康状况。