Tang L K, Martellock A C, Horiuchi J K
Am J Physiol. 1982 Jun;242(6):E392-7. doi: 10.1152/ajpendo.1982.242.6.E392.
The relationship between 17 beta-estradiol (E2) stimulation of luteinizing hormone (LH) response to LH-releasing hormone (LHRH) and E2 effect on LHRH binding was examined in pituitary monolayer cultures prepared from female rats. E2 pretreatment significantly (P less than 0.05) augmented the LHRH-induced LH release to 158-180% of the non-E2-treated controls. The maximal E2-priming effect could be observed after 1 day of treatment. E2 treatment for 3 days stimulated [D-Ala6]luteinizing hormone-releasing hormone (LHRHa) binding to about 1.5-fold that of the non-E2-treated controls without affecting the dissociation constant of LHRH receptor (Kd = 4 X 10(-10) M). The stimulatory effect of E2 on cell proliferation as determined by [3H]thymidine incorporation was also observed 3 days after treatment. However, E2 stimulation of LH accumulation in the cultured cells could be detected as early as 4 h after treatment. These results indicate that E2-priming effect on pituitary LH response to LHRH is initially associated with an increase in cellular LH content and later associated with increases in LHRH binding and in an index of cell proliferation that may include the LH-producing cells.
在从雌性大鼠制备的垂体单层培养物中，研究了17β-雌二醇（E2）对促黄体生成素（LH）释放激素（LHRH）刺激的LH反应的影响以及E2对LHRH结合的作用。E2预处理显著（P<0.05）增强了LHRH诱导的LH释放，达到未用E2处理的对照的158%-180%。治疗1天后可观察到最大的E2启动效应。E2处理3天刺激[D-Ala6]促黄体生成素释放激素（LHRHa）结合至未用E2处理的对照的约1.5倍，而不影响LHRH受体的解离常数（Kd = 4×10^(-10) M）。治疗3天后也观察到E2对细胞增殖的刺激作用，这是通过[3H]胸苷掺入法测定的。然而，E2刺激培养细胞中LH积累最早可在治疗后4小时检测到。这些结果表明，E2对垂体对LHRH的LH反应的启动效应最初与细胞内LH含量增加有关，随后与LHRH结合增加以及可能包括产生LH的细胞在内的细胞增殖指数增加有关。
