Maberly G F, Eastman C J, Smith H C, Waite K, Wilke T J
Aust J Exp Biol Med Sci. 1982 Feb;60(Pt 1):113-5. doi: 10.1038/icb.1982.10.
Nuclear protein extracts from mononuclear cells in normal subjects, subjects with the clinical syndrome of thyroid hormone resistance and cultured B lymphocytes bound thyroxine (T4) Ka; 1 . 3-3 . 5 X 10(9) m-1) and triiodothyronine (T3) (Ka; 1 . 2-3 . 9 X 10(9) m-1) with similar affinities. When an extra wash step was introduced, the maximum specific binding of T4 and T3 was reduced by 75% and binding was abolished by three washes. These data suggest binding is to a serum protein contaminant present in the mononuclear cell preparation.
正常受试者、患有甲状腺激素抵抗临床综合征的受试者以及培养的B淋巴细胞中的单核细胞核蛋白提取物以相似的亲和力结合甲状腺素（T4，解离常数Ka为1.3 - 3.5×10⁹ m⁻¹）和三碘甲状腺原氨酸（T3，解离常数Ka为1.2 - 3.9×10⁹ m⁻¹）。当增加一个额外的洗涤步骤时，T4和T3的最大特异性结合降低了75%，经过三次洗涤后结合被消除。这些数据表明这种结合是针对单核细胞制剂中存在的一种血清蛋白污染物。
