细胞色素c还原的动力学研究。与二羟基共轭化合物（儿茶酚和对苯二酚）的反应。
Kinetic studies on the reduction of cytochrome c. Reaction with dihydroxy conjugated compounds (catechols and quinols).
作者信息
Saleem M M, Wilson M T
出版信息
Biochem J. 1982 Mar 1;201(3):433-44. doi: 10.1042/bj2010433.
Abstract
The kinetics of reduction of cytochrome c by catechol(s), quinol(s) and related compounds were investigated by stopped-flow spectrophotometry. Studies on the influence of pH on the rates indicate that only deprotonated forms of these compounds act as reducing agents, with the dianionic forms being the most effective. The pH-independent second-order rate constants are reported. Hammett treatment of the effects of substituents on the aromatic ring structure of the reductants show that for electron transfer to occur the charge on the deprotonated species must not be withdrawn on to the substituents. Possible sites for electron donation to cytochrome c are discussed, and the results indicate that the haem edge is a likely candidate.
摘要
采用停流分光光度法研究了儿茶酚、对苯二酚及相关化合物还原细胞色素c的动力学。关于pH对反应速率影响的研究表明，只有这些化合物的去质子化形式可作为还原剂，其中二价阴离子形式最为有效。报告了与pH无关的二级速率常数。通过哈米特方程处理取代基对还原剂芳香环结构的影响表明，为了发生电子转移，去质子化物种上的电荷不能转移到取代基上。讨论了向细胞色素c供电子的可能位点，结果表明血红素边缘可能是一个候选位点。
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本文引用的文献
1
SPECTRA OF CYTOCHROME C MONOMER AND POLYMERS.细胞色素C单体和聚合物的光谱
Biochim Biophys Acta. 1963 Aug 6;73:641-3. doi: 10.1016/0006-3002(63)90334-2.
2
Interconversion of horse heart cytochrome C monomer and polymers.马心脏细胞色素C单体与聚合物的相互转化。
J Biol Chem. 1962 Nov;237:3397-405.
3
Preparation of crystalline Pseudomonas cvtochrome c-551 and its general properties.结晶假单胞菌细胞色素c-551的制备及其一般性质。
Biochem J. 1960 Oct;77(1):194-201. doi: 10.1042/bj0770194.
4
Specific colorimetric detection of o-diphenols and 3,4-dihydroxyphenylalanine-containing peptides.邻二酚和含3,4-二羟基苯丙氨酸肽的特异性比色检测
Anal Biochem. 1981 Feb;111(1):131-6. doi: 10.1016/0003-2697(81)90240-2.
5
Interaction of cytochrome c with phospholipid monolayers. Orientation and penetration of protein as functions of the packing density of film, nature of the phospholipids, and ionic content of the aqueous phase.细胞色素c与磷脂单层的相互作用。蛋白质的取向和渗透与膜的堆积密度、磷脂的性质以及水相离子含量的关系。
Biochemistry. 1981 Mar 17;20(6):1554-60. doi: 10.1021/bi00509a023.
6
Use of specific lysine modifications to identify the site of reaction between cytochrome c and ferricyanide.利用特定赖氨酸修饰来确定细胞色素c与铁氰化物之间的反应位点。
J Biol Chem. 1981 Feb 25;256(4):1611-5.
7
The arginines of cytochrome c. The reduction-binding site for 2,3-butanedione and ascorbate.细胞色素c的精氨酸。2,3-丁二酮和抗坏血酸的还原结合位点。
J Biol Chem. 1980 Dec 10;255(23):11094-7.
8
Kinetics of the reduction of horse heart ferricytochrome c. Ascorbate reduction in the presence and absence of urea.马心铁细胞色素c还原的动力学。在有和没有尿素存在的情况下抗坏血酸的还原作用。
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9
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Biochim Biophys Acta. 1980 Oct 3;592(3):506-18. doi: 10.1016/0005-2728(80)90095-x.
10
Evidence for the existence of two functionally distinct forms cytochrome c manomer at alkaline pH.在碱性pH条件下存在两种功能不同形式的细胞色素c单体的证据。
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